DEVELOPMENT OF AN ETHANOL-SELECTIVE OPTODE MEMBRANE BASED ON A REVERSIBLE CHEMICAL RECOGNITION PROCESS

Lipophilic trifluoroacetophenone derivatives incorporated in plasticized PVC membranes are able to selectively extract water and alcohols from the sample solution into the organic membrane phase, reversibly forming hydrates and hemi-acetals, respectively. Since this is accompanied by a change in the absorption spectrum of the acetophenone isologue, the chemical recognition process can directly be translated into an optical signal. With N-acetyl-N-dodecyl-4-trifluoroacetylaniline (ETH 6022) as the electrically neutral, lipophilized carrier ethanol can be determined from 0.5 to 35% (v/v) in aqueous solutions. The calibration curve for different ethanol-water mixtures shows a good correlation with the mathematically derived formalism and thus confirms the theoretically expected behavior. Besides high reproducibility of the optical signals, very short response times of less than 30 s were realized. The optode membrane presented exhibits a preference for ethanol compared to water by a factor greater than 11. The selectivities for several primary alcohols, such as methanol, ethanol, 1-propanol and 1-n-butanol, are comparable, but isopropanol and tert.-butanol are rejected by a factor of about 10. The alcohol concentration in different beverages was determined to evaluate the reliability of the system. The values obtained for wine, beer and different spirits show an excellent correlation with those obtained by a conventional approach involving distillation and density measurements. A residual standard deviation of +/- 0.27% (v/v) over the 0.7-40% (v/v) range was found.