A MODEL FOR BETA-GALACTOSIDASE PRODUCTION WITH A RECOMBINANT YEAST SACCHAROMYCES-CEREVISIAE IN FED-BATCH CULTURE

被引：15

作者：

HARDJITO, L ^{[1
]}

GREENFIELD, PF ^{[1
]}

LEE, PL ^{[1
]}

机构：

[1] UNIV QUEENSLAND, DEPT CHEM ENGN, ST LUCIA, QLD 4072, AUSTRALIA

来源：

BIOTECHNOLOGY PROGRESS | 1992年 / 8卷 / 04期

关键词：

D O I：

10.1021/bp00016a006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An unstructured model is developed to describe the growth and product formation behavior of a recombinant yeast Saccharomyces cerevisiae using beta-galactosidase as a model protein. The model shows good agreement with the experimental data over a range of conditions. It also accurately predicts the effect of growth rate on yield coefficient and gene expression. The simplicity and accuracy of the model make it suitable for designing and implementing control and optimization strategies for the production of recombinant proteins at large scale.

引用

页码：298 / 306

页数：9

共 46 条

[1] AN ALGORITHM FOR OPERATING A FED-BATCH FERMENTER AT OPTIMUM SPECIFIC-GROWTH RATE [J].

AGRAWAL, P ;

KOSHY, G ;

RAMSEIER, M .

BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (01) :115-125

[2] FED BATCH CULTURE OF SACCHAROMYCES-CEREVISIAE - PERSPECTIVE OF COMPUTER CONTROL TO ENHANCE PRODUCTIVITY IN BAKERS-YEAST CULTIVATION [J].

AIBA, S ;

NAGAI, S ;

NISHIZAWA, Y .

BIOTECHNOLOGY AND BIOENGINEERING, 1976, 18 (07) :1001-1016

[3] KINETICS OF PRODUCT INHIBITION IN ALCOHOL FERMENTATION [J].

AIBA, S ;

NAGATANI, M .

BIOTECHNOLOGY AND BIOENGINEERING, 1968, 10 (06) :845-&

[4] RESPIRATORY EFFICIENCY AND METABOLITE PARTITIONING AS REGULATORY PHENOMENA IN YEASTS [J].

ALEXANDER, MA ;

JEFFRIES, TW .

ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (01) :2-19

[5] EXAMINATION OF THE CRABTREE EFFECT IN SACCHAROMYCES-CEREVISIAE - ROLE OF RESPIRATORY ADAPTATION [J].

BARFORD, JP ;

HALL, RJ .

JOURNAL OF GENERAL MICROBIOLOGY, 1979, 114 (OCT) :267-275

[6] A MATHEMATICAL-MODEL FOR THE AEROBIC GROWTH OF SACCHAROMYCES-CEREVISIAE WITH A SATURATED RESPIRATORY CAPACITY [J].

BARFORD, JP ;

HALL, RJ .

BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (08) :1735-1762

[7] A GENERAL-MODEL FOR AEROBIC YEAST GROWTH - BATCH GROWTH [J].

BARFORD, JP .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (09) :907-920

[8]

Barnett JA, 1983, YEASTS CHARACTERISTI

[9] A NOVEL STRUCTURED KINETIC MODELING APPROACH FOR THE ANALYSIS OF PLASMID INSTABILITY IN RECOMBINANT BACTERIAL CULTURES [J].

BENTLEY, WE ;

KOMPALA, DS .

BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (01) :49-61

[10] PLASMID-ENCODED PROTEIN - THE PRINCIPAL FACTOR IN THE METABOLIC BURDEN ASSOCIATED WITH RECOMBINANT BACTERIA [J].

BENTLEY, WE ;

MIRJALILI, N ;

ANDERSEN, DC ;

DAVIS, RH ;

KOMPALA, DS .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (07) :668-681

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