EFFECT OF THERMODYNAMIC WATER ACTIVITY ON AMINO-ACID ESTER SYNTHESIS CATALYZED BY AGAROSE-CHYMOTRYPSIN IN 3-PENTANONE

Chymotrypsin linked to agarose beads by multi-point covalent attachment catalyzes synthesis of Ac-Trp-OEt in 3-pentanone even when the thermodynamic water activity (a(w)) of the system is reduced to as low as 0.4. If fully hydrated catalyst is added to the reaction mixture before removal of water, product is formed linearly once a(w) has stabilized. The initial rate is reduced from that if a(w) is kept close to 1 (0.47 mmol s-1 (kg enzyme)-1), to 50% (a(w) 0.9), 25% (a(w) 0.4) and < 1% (a(w) 0.25). The large drop between a(w) of 1 and 0.9 probably reflects the effects of water removal on the agarose gel structure. Catalyst partly dried (even only to a(w) 0.86) before adding to the organic phase is inactive. At reduced aw, the equilibrium (when reached) is shifted in favor of the ester, as expected.