学术探索
学术期刊
新闻热点
数据分析
智能评审

PURIFICATION AND CHARACTERIZATION OF CYCLODEXTRIN GLUCANOTRANSFERASE FROM AN ALKALOPHILIC BACILLUS SPECIES AND TRANSGLYCOSYLATION AT ALKALINE PHS

被引：35
作者：
KOMETANI, T
TERADA, Y
NISHIMURA, T
TAKII, H
OKADA, S
机构：
[1] Biochemical Research Laboratory, Ezaki Glico Co., Ltd., Osaka, Utajima 4-6-5, Nishiyodogawa
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 03期
关键词：
D O I：
10.1271/bbb.58.517
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cyclodextrin glucanotransferase (1,4-alpha-D-glucan 4-alpha-D-(1,4-alpha-D-glucano)-transferase (cyclizing), EC 2.4.1.19, CGTase) from an alkalophilic Bacillus species was purified by starch adsorption and Q-Sepharose chromatography. The purified enzyme had cyclizing activity, transglycosylating (coupling) activity, and starch-hydrolyzing activity, and their pH-activity curves had a single peak (pH 5.5 as the optimum pH) with a broad shoulder at alkaline pHs. Transglycosylation to various saccharides and flavonoids at alkaline pH was more effective than that at neutral pH. Among flavonoids, those containing rutinose (diosmin and hesperidin) were transglycosylated more effectively than those containing neohesperidose (naringin and neohesperidin).
引用
收藏
页码：517 / 520
页数：4
相关论文
共 21 条
[11]   TASTE AND STRUCTURE IN PHENOLIC GLYCOSIDES [J].
HOROWITZ, RM ;
GENTILI, B .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1969, 17 (04) :696-&
[12]  
KAMIYA S, 1969, SHIN BITAMIN GAKU, P439
[13]   ACCEPTOR SPECIFICITY OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS-STEAROTHERMOPHILUS AND SYNTHESIS OF ALPHA-D-GLUCOSYL O-BETA-D-GALACTOSYL-(1-]4)-BETA-D-GLUCOSIDE [J].
KITAHATA, S ;
HARA, K ;
FUJITA, K ;
NAKANO, H ;
KUWAHARA, N ;
KOIZUMI, K .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (09) :1386-1391
[14]   STUDIES ON CYCLODEXTRIN GLYCOSYLTRANSFERASE .3. TRANSFER ACTION OF CYCLODEXTRIN GLYCOSYLTRANSFERASE ON STARCH [J].
KITAHATA, S ;
OKADA, S .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1975, 39 (11) :2185-2191
[15]  
KITAHATA S, 1988, HDB AMYLASES RELATED, P154
[16]  
Krieg N. R., 1984, BERGEYS MANUAL SYSTE
[17]   PURIFICATION AND PROPERTIES OF CYCLODEXTRIN GLYCOSYLTRANSFERASE OF AN ALKALOPHILIC BACILLUS SP [J].
NAKAMURA, N ;
HORIKOSHI, K .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1976, 40 (05) :935-941
[18]   PURIFICATION AND PROPERTIES OF NEUTRAL-CYCLODEXTRIN GLYCOSYL-TRANSFERASE OF AN ALKALOPHILIC BACILLUS SP [J].
NAKAMURA, N ;
HORIKOSHI, K .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1976, 40 (09) :1785-1791
[19]   PURIFICATION AND CHARACTERIZATION OF CYCLODEXTRIN GLUCANOTRANSFERASE FROM AN ALKALOPHILIC BACTERIUM OF TAIWAN [J].
NOMOTO, M ;
CHEN, CC ;
SHEU, DC .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (11) :2701-2707
[20]   FURTHER STUDY ON THE 1,4-ALPHA-TRANSGLUCOSYLATION OF RUBUSOSIDE, A SWEET STEVIOL-BISGLUCOSIDE FROM RUBUS-SUAVISSIMUS [J].
OHTANI, K ;
AIKAWA, Y ;
ISHIKAWA, H ;
KASAI, R ;
KITAHATA, S ;
MIZUTANI, K ;
DOI, S ;
NAKAURA, M ;
TANAKA, O .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1991, 55 (02) :449-453
123