CLONING AND EXPRESSION OF THE LYSOSTAPHIN GENE IN BACILLUS-SUBTILIS AND LACTOBACILLUS-CASEI

被引:24
作者
GAIER, W [1 ]
VOGEL, RF [1 ]
HAMMES, WP [1 ]
机构
[1] UNIV HOHENHEIM,INST LEBENSMITTELTECHNOL,GARBENSTR 25,W-7000 STUTTGART 70,GERMANY
关键词
D O I
10.1111/j.1472-765X.1992.tb00651.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The lysostaphin structural gene was cloned in Bacillus subtilis DSM402 and in Lactobacillus casei 102S. The gene was expressed in both organisms and active lysostaphin was released into the medium. Lysostaphin produced by these organisms induced lysis of growing and heat inactivated staphylococci. Expression in a protective starter organism is a prerequisite to produce lysostaphin in situ in fermenting foods and hence, to reduce the hygienical risk of staphylococcal food poisoning.
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页码:72 / 76
页数:5
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