SIMIAN-VIRUS 40 SMALL-T ANTIGEN BINDS 2 ZINC IONS

被引：24

作者：

TURK, B

PORRAS, A

MUMBY, MC

RUNDELL, K

机构：

[1] NORTHWESTERN UNIV, DEPT MICROBIOL IMMUNOL, 303 E CHICAGO AVE, CHICAGO, IL 60611 USA

[2] UNIV TEXAS, SW MED CTR, DEPT PHARMACOL, DALLAS, TX 75235 USA

来源：

JOURNAL OF VIROLOGY | 1993年 / 67卷 / 06期

关键词：

D O I：

10.1128/JVI.67.6.3671-3673.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Six cysteine residues of the simian virus 40 small-t antigen (small-t) are important for stability of the protein. Stability has been shown to be related to the ability of small-t to bind zinc ions in vitro. Purified small-t expressed either in bacteria or from baculovirus vectors binds two molecules of zinc per molecule of protein. Thus, small-t may resemble GAL4, which contains a Zn(II)2Cys6 binuclear cluster.

引用

收藏

页码：3671 / 3673

页数：3

相关论文

共 23 条

[1] INTERACTION OF SIMIAN VIRUS-40 SMALL-T ANTIGEN PRODUCED IN BACTERIA WITH 56K-PROTEINS AND 32K-PROTEINS OF ANIMAL-CELLS [J].

BOSSERT, A ;

MULGAONKAR, P ;

RUNDELL, K .

JOURNAL OF VIROLOGY, 1985, 56 (01) :325-327

[2] NEW REGION OF SIMIAN VIRUS-40 GENOME REQUIRED FOR EFFICIENT VIRAL TRANSFORMATION [J].

BOUCK, N ;

BEALES, N ;

SHENK, T ;

BERG, P ;

DIMAYORCA, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (05) :2473-2477

[3] SIMIAN VIRUS-40 (SV40) SMALL T-ANTIGEN INHIBITS SV40 DNA-REPLICATION INVITRO [J].

CARBONE, M ;

HAUSER, J ;

CARTY, MP ;

RUNDELL, K ;

DIXON, K ;

LEVINE, AS .

JOURNAL OF VIROLOGY, 1992, 66 (03) :1804-1808

[4] DIFFERENTIAL REQUIREMENT FOR SV40 EARLY GENES IN IMMORTALIZATION AND TRANSFORMATION OF PRIMARY RAT AND HUMAN-EMBRYONIC CELLS [J].

CHANG, LS ;

PAN, S ;

PATER, MM ;

DIMAYORCA, G .

VIROLOGY, 1985, 146 (02) :246-261

[5] CALCULATION OF PROTEIN EXTINCTION COEFFICIENTS FROM AMINO-ACID SEQUENCE DATA [J].

GILL, SC ;

VONHIPPEL, PH .

ANALYTICAL BIOCHEMISTRY, 1989, 182 (02) :319-326

[6] EFFECT OF ZINC IONS ON THE BIOCHEMICAL BEHAVIOR OF SIMIAN VIRUS-40 SMALL-T ANTIGEN EXPRESSED IN BACTERIA [J].

GOSWAMI, R ;

TURK, B ;

ENDERLE, K ;

HOWE, A ;

RUNDELL, K .

JOURNAL OF VIROLOGY, 1992, 66 (03) :1746-1751

[7] MONOCLONAL-ANTIBODIES SPECIFIC FOR SIMIAN VIRUS-40 TUMOR-ANTIGENS [J].

HARLOW, E ;

CRAWFORD, LV ;

PIM, DC ;

WILLIAMSON, NM .

JOURNAL OF VIROLOGY, 1981, 39 (03) :861-869

[8] MUTATIONAL ANALYSIS OF SIMIAN VIRUS-40 SMALL-T ANTIGEN [J].

JOG, P ;

JOSHI, B ;

DHAMANKAR, V ;

IMPERIALE, MJ ;

RUTILA, J ;

RUNDELL, K .

JOURNAL OF VIROLOGY, 1990, 64 (06) :2895-2900

[9] THE ZINC FINGER REGION OF SIMIAN VIRUS-40 LARGE T-ANTIGEN [J].

LOEBER, G ;

PARSONS, R ;

TEGTMEYER, P .

JOURNAL OF VIROLOGY, 1989, 63 (01) :94-100

[10] ROLES OF THE SIMIAN VIRUS-40 TUMOR-ANTIGENS IN TRANSFORMATION OF CHINESE-HAMSTER LUNG-CELLS [J].

MARTIN, RG ;

SETLOW, VP ;

EDWARDS, CAF ;

VEMBU, D .

CELL, 1979, 17 (03) :635-643