BACILLUS-STEAROTHERMOPHILUS PLASMID PSTK1 REPLICON IS FUNCTIONAL IN ESCHERICHIA-COLI

被引：4

作者：

NARUMI, I

NAKAYAMA, N

NAKAMOTO, S

KIHARA, H

机构：

[1] NEC CORP LTD,MIYAMAE KU,KAWASAKI,KANAGAWA 216,JAPAN

[2] KANSAI MED UNIV,DEPT LIBERAL ARTS,PHYS LAB,HIRAKATA,OSAKA 573,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 05期

关键词：

D O I：

10.1007/BF00132013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A kanamycin-resistant plasmid possessing a thermostable replicon derived from Bacillus stearothermophilus cryptic plasmid pSTK1 was constructed. The plasmid could transform not only B. stearothermophilus and Bacillus subtilis, but also Gram-negative Escherichia coli. The behavior of the plasmid in the hosts was examined. The plasmid was stably maintained even at 67 degrees C in B. stearothermophilus without selective pressure. During the plasmid replication, single-stranded DNA (ssDNA) intermediates were found in E. coli, while these were not found in B. subtilis.

引用

收藏

页码：475 / 480

页数：6

相关论文

共 32 条

[21]

OSKAM L, 1992, M63891 GENB ACC NO

[22] PROTEIN-DNA RECOGNITION [J].

PABO, CO ;

SAUER, RT .

ANNUAL REVIEW OF BIOCHEMISTRY, 1984, 53 :293-321

[23] DNA-SEQUENCE AND ANALYSIS OF A CRYPTIC 4.2-KB PLASMID FROM THE FILAMENTOUS CYANOBACTERIUM, PLECTONEMA SP STRAIN PCC-6402 [J].

PERKINS, DR ;

BARNUM, SR .

PLASMID, 1992, 28 (02) :170-176

[24]

Sambrook J., 1989, MOL CLONING LAB MANU

[25] TRANSFORMATION OF CORYNEBACTERIUM-DIPHTHERIAE, CORYNEBACTERIUM-ULCERANS, CORYNEBACTERIUM-GLUTAMICUM, AND ESCHERICHIA-COLI WITH THE C-DIPHTHERIAE PLASMID PNG2 [J].

SERWOLDDAVIS, TM ;

GROMAN, N ;

RABIN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4964-4968

[26] STRUCTURAL ORGANIZATION OF A CRYPTIC PLASMID, PMA1, FROM MICROCYSTIS-AERUGINOSA F AERUGINOSA KUTZING [J].

TOMINAGA, H ;

SOEJIMA, K ;

KAWAGISHI, S ;

ASHIDA, H ;

SAWA, Y ;

OCHIAI, H .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (09) :1503-1507

[27] GENETIC-ANALYSIS OF A LACTOCOCCAL PLASMID REPLICON [J].

XU, FF ;

PEARCE, LE ;

YU, PL .

MOLECULAR & GENERAL GENETICS, 1991, 227 (01) :33-39

[28] A SMALL PLASMID, PCA2.4, FROM THE CYANOBACTERIUM SYNECHOCYSTIS SP STRAIN PCC-6803 ENCODES A REP PROTEIN AND REPLICATES BY A ROLLING CIRCLE MECHANISM [J].

YANG, XY ;

MCFADDEN, BA .

JOURNAL OF BACTERIOLOGY, 1993, 175 (13) :3981-3991

[29] IMPROVED M13 PHAGE CLONING VECTORS AND HOST STRAINS - NUCLEOTIDE-SEQUENCES OF THE M13MP18 AND PUC19 VECTORS [J].

YANISCHPERRON, C ;

VIEIRA, J ;

MESSING, J .

GENE, 1985, 33 (01) :103-119

[30] ROLLING-CIRCLE REPLICATION OF THE PLASMID PKYM ISOLATED FROM A GRAM-NEGATIVE BACTERIUM [J].

YASUKAWA, H ;

HASE, T ;

SAKAI, A ;

MASAMUNE, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10282-10286

← 1 2 3 4 →