BACILLUS-STEAROTHERMOPHILUS PLASMID PSTK1 REPLICON IS FUNCTIONAL IN ESCHERICHIA-COLI

被引：4

作者：

NARUMI, I

NAKAYAMA, N

NAKAMOTO, S

KIHARA, H

机构：

[1] NEC CORP LTD,MIYAMAE KU,KAWASAKI,KANAGAWA 216,JAPAN

[2] KANSAI MED UNIV,DEPT LIBERAL ARTS,PHYS LAB,HIRAKATA,OSAKA 573,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 05期

关键词：

D O I：

10.1007/BF00132013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A kanamycin-resistant plasmid possessing a thermostable replicon derived from Bacillus stearothermophilus cryptic plasmid pSTK1 was constructed. The plasmid could transform not only B. stearothermophilus and Bacillus subtilis, but also Gram-negative Escherichia coli. The behavior of the plasmid in the hosts was examined. The plasmid was stably maintained even at 67 degrees C in B. stearothermophilus without selective pressure. During the plasmid replication, single-stranded DNA (ssDNA) intermediates were found in E. coli, while these were not found in B. subtilis.

引用

页码：475 / 480

页数：6

共 32 条

[11] CONSERVED SEQUENCE MOTIFS IN THE INITIATOR PROTEINS FOR ROLLING CIRCLE DNA-REPLICATION ENCODED BY DIVERSE REPLICONS FROM EUBACTERIA, EUKARYOTES AND ARCHAEBACTERIA [J].