RAPID COLORIMETRIC ASSAY FOR THE QUANTIFICATION OF LEUKEMIA INHIBITORY FACTOR (LIF) AND INTERLEUKIN-6 (IL-6)

被引：259

作者：

OHNO, M ^{[1
]}

ABE, T ^{[1
]}

机构：

[1] ASAHI CHEM IND CO LTD, LIFE SCI RES LABS, SHIZUOKA, JAPAN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 145卷 / 1-2期

关键词：

COLORIMETRIC ASSAY; LEUKEMIA INHIBITORY FACTOR (LIF); DIFFERENTIATION INDUCING FACTOR (DIF); TETRAZOLIUM SALT; MTT; INTERLEUKIN-6;

D O I：

10.1016/0022-1759(91)90327-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The methods in general use for quantifying leukemia inhibitory factor (LIF) activity involve measurements of suppression of proliferation or stimulation of phagocytic activity of mouse myelocytic leukemia (M1) cells. We have developed a novel assay for LIF activity which consists of measuring MTT reduction by M1 cells. The ability of M1 cells to reduce MTT correlates with the level of LIF used for stimulation. The MTT assay of LIF is much quicker than previously used quantitation methods and it is just as sensitive. The MTT assay can also be used to quantify IL-6.

引用

页码：199 / 203

页数：5

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