SEA BASS (DICENTRARCHUS-LABRAX L) VITELLOGENIN .2. VALIDATION OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)

被引：92

作者：

MANANOS, E

NUNEZ, J

ZANUY, S

CARRILLO, M

LEMENN, F

机构：

[1] INST ACUICULTURA TORRE DE LA SAL, Castellon de La Plana, SPAIN

[2] ORSTOM, CTR RECH OCEANOL, ABIDJAN, COTE IVOIRE

[3] UNIV BORDEAUX 1, BIOL MARINE LAB, F-33405 TALENCE, FRANCE

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1994年 / 107卷 / 02期

关键词：

DICENTRARCHUS LABRAX L; VITELLOGENIN;

D O I：

10.1016/0305-0491(94)90042-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a competitive ELISA was developed for the quantification of vitellogenin (VTG) in sea bass. The purity of the antigen (VTG) and the specificity of the antibody (AbVTG) used in the assay, were assessed by ELISA and radial immunodiffusion, and no crossreactivity was observed with the male plasma. The sensitivity range of the ELISA was 1-60 ng/ml (85-20% of binding). The intra- and inter-assay variations (at 50% of binding) were 5.3% (N = 5) and 9.8% (N = 9), respectively. Annual VTG levels were quantified in animals reared under fish farming conditions and the VTG levels were correlated with the oocyte development.

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页码：217 / 223

页数：7

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