STRUCTURES OF THE ASPARAGINE-LINKED SUGAR CHAIN OF GLUCOSE TRANSPORTER FROM HUMAN ERYTHROCYTES

被引：19

作者：

ENDO, T

KASAHARA, M

KOBATA, A

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT BIOCHEM,TOKYO 108,JAPAN

[2] TEIKYO UNIV,SCH MED,BIOPHYS LAB,HACHIOJI,TOKYO 19203,JAPAN

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 39期

关键词：

D O I：

10.1021/bi00491a005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The asparagine-linked sugar chain of glucose transporter from human erythrocytes was quantitatively released as oligosaccharides from the polypeptide backbone by hydrazinolysis. They were converted to radioactive oligosaccharides by NaB3H4reduction after N-acetylation and fractionated by anion-exchange column chromatography and Bio-Gel P-4 column chromatography after sialidase treatment. Structural study of each oligosaccharide by exo-and endoglycosidase digestion and methylation analysis indicated that the glycoprotein contains a high-mannose-type oligosaccharide, Man9·GlcNAc·GlcNAc, and biantennary complex-type oligosaccharides with Manα1→6(±GlcNAcβ1→4)(Manα1→3)Manβ1→ 4GlcNAcβ1→4(±Fucα1→6)GlcNAc as their cores and the poly-N-acetyllactosamine composed of about 16 N-acetyllactosaminyl units as their outer chains. These structural features of the sugar moiety of glucose transporter are quite different from those of two major intrinsic glycoproteins of human erythrocytes, glycophorin A and band 3. © 1990, American Chemical Society. All rights reserved.

引用

页码：9126 / 9134

页数：9

共 49 条

[11] GLASGOW LR, 1977, J BIOL CHEM, V252, P8615
[12] MONOSACCHARIDE TRANSPORTER FROM HUMAN-ERYTHROCYTES IS HETEROGENEOUSLY GLYCOSYLATED
GORGA, FR
BALDWIN, SA
LIENHARD, GE
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 91 (03) : 955 - 961
[13] ELUCIDATION OF THE PHENOTYPIC CHANGE ON THE SURFACE OF HAD-1 CELL, A MUTANT-CELL LINE OF MOUSE FM3A CARCINOMA-CELLS SELECTED BY RESISTANCE TO NEWCASTLE-DISEASE VIRUS-INFECTION
HARA, T
ENDO, T
FURUKAWA, K
KAWAKITA, M
KOBATA, A
[J]. JOURNAL OF BIOCHEMISTRY, 1989, 106 (02) : 236 - 247
[14] SYNTHESIS AND PROCESSING OF ASPARAGINE-LINKED OLIGOSACCHARIDES
HUBBARD, SC
IVATT, RJ
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1981, 50 : 555 - 583
[15] ITO M, 1986, J BIOL CHEM, V261, P14278
[16] KASAHARA M, 1977, J BIOL CHEM, V252, P7384
[17] RECONSTITUTION OF D-GLUCOSE TRANSPORT CATALYZED BY A PROTEIN-FRACTION FROM HUMAN ERYTHROCYTES IN SONICATED LIPOSOMES
KASAHARA, M
HINKLE, PC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (02) : 396 - 400
[18] THE STRUCTURE OF THE ASPARAGINE-LINKED SUGAR CHAINS OF BOVINE BRAIN RIBONUCLEASE
KATOH, H
OHGI, K
IRIE, M
ENDO, T
KOBATA, A
[J]. CARBOHYDRATE RESEARCH, 1990, 195 (02) : 273 - 293
[19] KITAMIKADO M, 1982, METHOD ENZYMOL, V83, P619
[20] KOCHIBE N, 1989, J BIOL CHEM, V264, P173

← 1 2 3 4 5 →