STRUCTURES OF THE ASPARAGINE-LINKED SUGAR CHAIN OF GLUCOSE TRANSPORTER FROM HUMAN ERYTHROCYTES

被引：19

作者：

ENDO, T

KASAHARA, M

KOBATA, A

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT BIOCHEM,TOKYO 108,JAPAN

[2] TEIKYO UNIV,SCH MED,BIOPHYS LAB,HACHIOJI,TOKYO 19203,JAPAN

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 39期

关键词：

D O I：

10.1021/bi00491a005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The asparagine-linked sugar chain of glucose transporter from human erythrocytes was quantitatively released as oligosaccharides from the polypeptide backbone by hydrazinolysis. They were converted to radioactive oligosaccharides by NaB3H4reduction after N-acetylation and fractionated by anion-exchange column chromatography and Bio-Gel P-4 column chromatography after sialidase treatment. Structural study of each oligosaccharide by exo-and endoglycosidase digestion and methylation analysis indicated that the glycoprotein contains a high-mannose-type oligosaccharide, Man9·GlcNAc·GlcNAc, and biantennary complex-type oligosaccharides with Manα1→6(±GlcNAcβ1→4)(Manα1→3)Manβ1→ 4GlcNAcβ1→4(±Fucα1→6)GlcNAc as their cores and the poly-N-acetyllactosamine composed of about 16 N-acetyllactosaminyl units as their outer chains. These structural features of the sugar moiety of glucose transporter are quite different from those of two major intrinsic glycoproteins of human erythrocytes, glycophorin A and band 3. © 1990, American Chemical Society. All rights reserved.

引用

页码：9126 / 9134

页数：9

共 49 条

[41] TURCO SJ, 1980, J BIOL CHEM, V255, P3266
[42] YAMASHITA K, 1985, J BIOL CHEM, V260, P4688
[43] AN ALPHA-MANNOSIDASE PURIFIED FROM ASPERGILLUS-SAITOI IS SPECIFIC FOR ALPHA-1,2 LINKAGES
YAMASHITA, K
ICHISHIMA, E
ARAI, M
KOBATA, A
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 96 (03) : 1335 - 1342
[44] SUBSTRATE-SPECIFICITY OF DIPLOCOCCAL BETA-N-ACETYLHEXOSAMINIDASE, A USEFUL ENZYME FOR THE STRUCTURAL STUDIES OF COMPLEX TYPE ASPARAGINE-LINKED SUGAR CHAINS
YAMASHITA, K
OHKURA, T
YOSHIMA, H
KOBATA, A
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1981, 100 (01) : 226 - 232
[45] YAMASHITA K, 1982, METHOD ENZYMOL, V83, P105
[46] YAMASHITA K, 1984, J BIOL CHEM, V259, P834
[47] YOSHIMA H, 1980, J BIOL CHEM, V255, P793
[48] YOSHIMA H, 1980, J BIOL CHEM, V255, P9713
[49] THE ASPARAGINE-LINKED SUGAR CHAINS OF PLASMA-MEMBRANE GLYCOPROTEINS OF K-562 HUMAN-LEUKEMIC CELLS - A COMPARATIVE-STUDY WITH HUMAN-ERYTHROCYTES
YOSHIMA, H
SHIRAISHI, N
MATSUMOTO, A
MAEDA, S
SUGIYAMA, T
KOBATA, A
[J]. JOURNAL OF BIOCHEMISTRY, 1982, 91 (01) : 233 - 246

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