THE DETECTION OF LIPID-PEROXIDATION PRODUCTS IN CRYOPROTECTED AND FROZEN RICE CELLS - CONSEQUENCES FOR POSTTHAW SURVIVAL

被引：47

作者：

BENSON, EE ^{[1
]}

LYNCH, PT ^{[1
]}

JONES, J ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,DEPT LIFE SCI,PLANT GENET MANIPULAT GRP,NOTTINGHAM NG7 2RD,ENGLAND

来源：

PLANT SCIENCE | 1992年 / 85卷 / 01期

关键词：

FREE RADICAL; GERMPLASM; MALONDIALDEHYDE; ORYZA-SATIVA; VIABILITY TEST; LIPID PEROXIDATION;

D O I：

10.1016/0168-9452(92)90099-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipid peroxidation was measured in frozen/thawed and unfrozen rice cells using the thiobarbituric acid (TBA) assay for malondialdehyde (MDA), a breakdown product of lipid peroxides. MDA was detected in increased amounts in frozen/thawed rice cells compared to unfrozen controls, suggesting that freezing injury can promote lipid peroxidation. The MDA content of rice cells was influenced by the carbon source (sucrose or fructose) in the recovery medium. Levels of peroxidation were greater in the presence of fructose.

引用

页码：107 / 114

页数：8

共 25 条

[1]

AMINO S, 1988, PLANT CELL PHYSIOL, V29, P483

[2]

Benson E.E., 1990, FREE RADICAL DAMAGE

[3]

BENSON EE, 1987, CRYO-LETT, V8, P35

[4]

BENSON EE, 1988, CRYO-LETT, V9, P120

[5] FREEZE-PRESERVATION OF RICE CELLS - A PHYSIOLOGICAL STUDY OF FREEZE-THAWED CELLS [J].

CELLA, R ;

COLOMBO, R ;

GALLI, MG ;

NIELSEN, E ;

ROLLO, F ;

SALA, F .

PHYSIOLOGIA PLANTARUM, 1982, 55 (03) :279-284

[6]

ESTERBAUER H, 1988, ISI ATLAS-BIOCHEM, V1, P311

[7]

FINCH RP, 1991, BIOTECHNOLOGY AGR FO, V14, P251

[8] CRYOPROTECTANT REMOVAL TEMPERATURE AS A FACTOR IN THE SURVIVAL OF FROZEN RICE AND SUGARCANE CELLS [J].

FINKLE, BJ ;

ULRICH, JM .

CRYOBIOLOGY, 1982, 19 (03) :329-335

[9] LIPID-PEROXIDATION MEASURED AS THIOBARBITURIC ACID-REACTIVE SUBSTANCES IN TISSUE-SLICES - CHARACTERIZATION AND COMPARISON WITH HOMOGENATES AND MICROSOMES [J].

FRAGA, CG ;

LEIBOVITZ, BE ;

TAPPEL, AL .

FREE RADICAL BIOLOGY AND MEDICINE, 1988, 4 (03) :155-161

[10]

Frankel E N, 1987, Free Radic Res Commun, V3, P213, DOI 10.3109/10715768709069786

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