CHROMATOGRAPHIC ISOLATION OF ANTIOXIDANTS GUIDED BY A METHYL LINOLEATE ASSAY

被引：33

作者：

BERTELSEN, G

CHRISTOPHERSEN, C

NIELSEN, PH

MADSEN, HL

STADEL, P

机构：

[1] UNIV COPENHAGEN,HC ORSTED INST,INST CHEM,DK-2100 COPENHAGEN,DENMARK

[2] ROYAL VET & AGR UNIV,DEPT DAIRY & FOOD SCI,KVL CTR FOOD RES,DK-1871 FREDERIKSBERG C,DENMARK

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1995年 / 43卷 / 05期

关键词：

ANTIOXIDANT ASSAY; ASSAY-GUIDED ISOLATION; ROSMARINIC ACID; SATUREJA HORTENSIS;

D O I：

10.1021/jf00053a027

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A convenient and fast method for establishing the relative antioxidant activity of chromatographically purified fractions of plant extracts has been developed. The assay electrochemically determines the myoglobin-catalyzed oxygen consumption following addition of the fractions to methyl linoleate. Since the oxygen consumption rate decreases with increasing antioxidant activity, the method can be used for systematic screening of naturally occurring antioxidants. Applying this method to extracts of Satureja hortensis led to isolation of rosmarinic acid.

引用

页码：1272 / 1275

页数：4

共 34 条

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[2]

CUVELIER ME, 1990, SCI ALIMENT, V10, P797

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