HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF SPECTINOMYCIN IN SWINE, CALF AND CHICKEN PLASMA USING POSTCOLUMN DERIVATIZATION

被引：26

作者：

HAAGSMA, N ^{[1
]}

SCHERPENISSE, P ^{[1
]}

SIMMONDS, RJ ^{[1
]}

WOOD, SA ^{[1
]}

REES, SA ^{[1
]}

机构：

[1] UPJOHN LABS EUROPE,CRAWLEY,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 672卷 / 01期

关键词：

D O I：

10.1016/0378-4347(95)00211-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An HPLC method for the determination of spectinomycin in swine, calf and chicken plasma at 0.1 mu g/ml or higher is described. The clean-up is based upon ion-pair solid-phase extraction on a High Hydrophobic C-18 column treated with sodium dioctyl sulfosuccinate. After elution with methanol, spectinomycin is chromatographed on a Spherisorb SCX column using 0.1 M sodium sulphate solution (pH 2.6)-acetonitrile (80:20, v/v) as mobile phase. Fluorescence detection is at an excitation wavelength of 340 nm and an emission wavelength of 460 nm after post-column oxidation with sodium hypochlorite followed by derivatization with o-phthaldialdehyde, Mean recoveries were 99+/-2% (n=6), 99+/-2% (n=7) and 104+/-2% (n=6) for swine, calf and chicken plasma, respectively, at the 0.1 mu g/ml level.

引用

页码：165 / 171

页数：7

共 9 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF SPECTINOMYCIN IN TURKEY PLASMA [J].