DIRECT APPLICATION TO DAIRY FOODS OF A LISTERIA-SPECIFIC OLIGONUCLEOTIDE PROBE TO 16S-RIBOSOMAL-RNA

被引：10

作者：

BROOKS, JL ^{[1
]}

BACK, JP ^{[1
]}

KROLL, RG ^{[1
]}

机构：

[1] INST FOOD RES, DEPT MICROBIOL, READING, ENGLAND

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1992年 / 16卷 / 04期

关键词：

LISTERIA; OLIGONUCLEOTIDE PROBE; 16S RIBOSOMAL-RNA; RNA EXTRACTION; DAIRY FOOD;

D O I：

10.1016/0168-1605(92)90032-X

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A potentially Listeria-specific 28 base oligonucleotide probe was designed from 16S rRNA sequence data. Using either P-32 or non-radioactive (alkaline phosphatase) labels, the probe was shown to be highly specific as it hybridised to RNA extracted from all of the species of Listeria but not to any of the other bacteria tested. Both probe methods were highly sensitive and ca 10(2) cfu/ml Listeria could be detected in pure cultures. A rapid procedure for extracting RNA from milk, Camembert and cottage cheese was developed. This allowed the direct application of the probe to these foods and gave a rapid and specific method of detecting > 10(2) cfu/g or ml Listeria in these foods.

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收藏

页码：303 / 312

页数：10

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