PROCEDURES FOR DETECTION OF DNA BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY USING A MODIFIED NAFION FILM SUBSTRATE

被引：27

作者：

BAI, J ^{[1
]}

LIN, YH ^{[1
]}

LIANG, XL ^{[1
]}

ZHU, YD ^{[1
]}

LUBMAN, DM ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 12期

关键词：

D O I：

10.1002/rcm.1290091217

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A Nafion film substrate modified with concentrated ammonia was shown to improve the detection of DNA using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), especially for DNA strands greater than 200 base pairs. Experimental procedures relating to the detection of DNA by MALDI-MS using this modified Nation substrate are described.

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页码：1172 / 1176

页数：5

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