MURINE HOMEOBOX-CONTAINING GENE, MSX-1 - ANALYSIS OF GENOMIC ORGANIZATION, PROMOTER STRUCTURE, AND POTENTIAL AUTOREGULATORY CIS-ACTING ELEMENTS

被引:33
作者
KUZUOKA, M
TAKAHASHI, T
GURON, C
RAGHOW, R
机构
[1] UNIV TENNESSEE, DEPT MED, MEMPHIS, TN 38104 USA
[2] UNIV TENNESSEE, DEPT PHARMACOL, MEMPHIS, TN USA
[3] VET AFFAIRS MED CTR, MEMPHIS, TN 38104 USA
关键词
D O I
10.1006/geno.1994.1228
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Detailed molecular organization of the coding and upstream regulatory regions of the murine homeodomain-containing gene, Msx-1, is reported. The protein-encoding portion of the gene is contained in two exons, 590 and 1214 bp in length, separated by a 2107-bp intron; the homeodomain is located in the second exon. The two-exon organization of the murine Msx-1 gene resembles a number of other homeodomain-containing genes. The 5'-(GTAAGT) and 3'-(CCCTAG) splicing junctions and the mRNA polyadenylation signal (UAUAA) of the murine Msx-1 gene are also characteristic of other vertebrate genes. By nuclease protection and primer extension assays, the start of transcription of the Msx-1 gene was located 256 bp upstream of the first AUG. Computer analysis of the promoter proximal 1280-bp sequence revealed a number of potentially important cis-regulatory sequences; these include the recognition elements for Ap-1, Ap-2, Ap-3, Sp-1, a possible binding site for RAR:RXR, and a number of TCF-1 consensus motifs. Importantly, a perfect reverse complement of (C/G)TTAATTG, which was recently shown to be an optimal binding sequence for the homeodomain of Msx-1 protein (K.M. Catron, N. ner, and C. Abate (1993) Mel. Cell. Biol. 13:2354-2365), was also located in the murine Msx-1 promoter. Binding of bacterially expressed Msx-1 homeodomain polypeptide to Msx-1-specific oligonucleotide was experimentally demonstrated, raising a distinct possibility of autoregulation of this developmentally regulated gene. (C) 1994 Academic Press, Inc.
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页码:85 / 91
页数:7
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