HYBRID LIVER SUPPORT SYSTEM IN A SHORT-TERM APPLICATION ON HEPATECTOMIZED PIGS

被引：41

作者：

GERLACH, J

TROST, T

RYAN, CJ

MEISSLER, M

HOLE, O

MULLER, C

NEUHAUS, P

机构：

[1] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,INST KLIN CHEM & BIOCHEM,D-14050 BERLIN,GERMANY

[2] HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DEPT SURG,LONDON,ENGLAND

来源：

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS | 1994年 / 17卷 / 10期

关键词：

HEPATOCYTE CULTURE; LIVER CELL PERFUSION SYSTEMS; BIOREACTORS; HYBRID LIVER SUPPORT THERAPY;

D O I：

10.1177/039139889401701007

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A short term application of a hybrid liver support system in circuits with continuous plasma-separation was investigated in a model of hepatectomized pigs under general anesthesia Primary pig hepatocytes were immobilized in a bioreactor with three independent capillary systems. An immune barrier is achieved by avoiding the direct contact of blood cells with the hepatocytes by a plasmaseparation step and by an outflow filtration within the reactor. In three groups (hepatectomized pigs and system with- or without hepatocytes as well as untreated pigs with system without hepatocytes) the short term metabolism of the reactors was positively demonstrated by investigating ammonia detoxification, phenylalanine- and lactate metabolism. Limitations of the presented model are discussed.

引用

页码：549 / 553

页数：5

共 22 条

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