PURIFICATION AND CHARACTERIZATION OF PECTIN ACETYLESTERASE FROM ORANGE PEEL

被引：36

作者：

WILLIAMSON, G

机构：

[1] AFRC Institute of Food Research, Norwich, NR4 7UA, Colney Lane

来源：

PHYTOCHEMISTRY | 1991年 / 30卷 / 02期

关键词：

CITRUS-SINENSIS; RUTACEAE; ORANGE PEEL; ENZYME PURIFICATION; ACETYLESTERASE; PECTIN; PECTIN ACETYLESTERASE; PECTIN METHYLESTERASE; PECTINESTERASE;

D O I：

10.1016/0031-9422(91)83701-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pectin acetylesterase was purified from orange peel using ammonium sulphate fractionation, followed by chromatography on Sephadex G-25 superfine, mono S and Superose 6. The purified enzyme has a M(r) of 29 000. It has no pectin methylesterase activity, but rapidly de-esterifies triacetin. The pectin acetylesterase activity is increased 4.8-fold by prior incubation of sugarbeet pectin with pectin methylesterase, which suggests that the acetylesterase preferentially deacetylates galacturonyl residues containing a free C-6 carboxy group.

引用

页码：445 / 449

页数：5

共 20 条

[1]

Abraham E P, 1975, Methods Enzymol, V43, P728

[2] DISTRIBUTION OF METHOXYL GROUPS IN PECTINS [J].