A METHOD FOR EXTRACTING INTACT RNA FROM FRUITS RICH IN POLYSACCHARIDES USING RIPE MANGO MESOCARP

被引：202

作者：

LOPEZGOMEZ, R

GOMEZLIM, MA

机构：

来源：

HORTSCIENCE | 1992年 / 27卷 / 05期

关键词：

MANGIFERA-INDICA; RNA EXTRACTION; MANGO; INVITRO TRANSLATION; FRUITS; NORTHERN BLOTS;

D O I：

10.21273/HORTSCI.27.5.440

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

A rapid and simple procedure is described for efficiently extracting intact RNA from mango (Mangifera indica L. cv. Manila) mesocarp, a tissue rich in polysaccharides. The RNA can be used for in vitro translation, northern blots, and cDNA synthesis. This method is applicable to other fleshy fruits rich in polysaccharides.

引用

页码：440 / 442

页数：3

共 19 条

[11] MEASUREMENT OF INCORPORATION OF RADIOACTIVE AMINO ACIDS INTO PROTEIN BY A FILTER-PAPER DISK METHOD [J].