IN-VIVO AGGREGATION OF MAIZE ACTIVATOR (AC) TRANSPOSASE IN NUCLEI OF MAIZE ENDOSPERM AND PETUNIA PROTOPLASTS

被引:45
作者
HEINLEIN, M [1 ]
BRATTIG, T [1 ]
KUNZE, R [1 ]
机构
[1] UNIV COLOGNE, INST GENET, D-50931 COLOGNE, GERMANY
关键词
D O I
10.1111/j.1365-313X.1994.00705.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The transposase (TPase) of the maize transposon Activator (Ac) accumulates in the nuclei of maize endosperm and transfected Petunia protoplasts, where it aggregates into rod-like structures about 2 mu m in length. In petunia protoplasts the amount of TPase aggregates increases with the strength of the promoter fused to the Ac-coding region. The excision frequency of a Ds element, however, does not increase proportionally. The data suggest that the aggregated TPase is not responsible for the mobilization of the Ds element, but rather is a transpositionally inactive form of the protein. In contrast to the full-length TPase, a functional, N-terminally truncated TPase derivative is inefficiently transported into the nucleus at high expression levels and aggregates predominantly in the cytoplasm. Accordingly, the N-terminus of the TPase is involved in nuclear localization and/or aggregation.
引用
收藏
页码:705 / 714
页数:10
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