ANALYSIS OF NUCLEOTIDE POOLS IN HUMAN LYMPHOMA-CELLS BY CAPILLARY ELECTROPHORESIS

被引：28

作者：

SHAO, XW

ONEILL, K

ZHAO, ZX

ANDERSON, S

MALIK, A

LEE, M

机构：

[1] BRIGHAM YOUNG UNIV,DEPT MICROBIOL,PROVO,UT 84602

[2] BRIGHAM YOUNG UNIV,DEPT CHEM,PROVO,UT 84602

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 680卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)85144-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrophoresis is applied to determine nucleotide pool levels in human Burkitt lymphoma cells. The analysis was performed on a 65 cm x 50 mu m I.D. Ucon-coated column with on-column UV detector. The method requires only nanoliters of sample and a simple sample preparation procedure. Over 12 nucleotides were separated and quantitated with high resolution and reproducibility. The whole capillary electrophoretic separation time was only 35 min, These results demonstrate that capillary electrophoresis provides a useful and easy way to analyze nucleotide pools in cells.

引用

页码：463 / 468

页数：6

共 29 条

[11]

HAVERBEKE DAV, 1978, J LIQ CHROMATOGR, V1, P507

[12] CELLULAR PHARMACOLOGY OF 2',3'-DIDEOXY-2',3'-DIDEHYDROTHYMIDINE, A NUCLEOSIDE ANALOG ACTIVE AGAINST HUMAN IMMUNODEFICIENCY VIRUS [J].