PROFILING TAXANES IN TAXUS EXTRACTS USING LC/MS AND LC/MS/MS TECHNIQUES

被引：60

作者：

KERNS, EH ^{[1
]}

VOLK, KJ ^{[1
]}

HILL, SE ^{[1
]}

LEE, MS ^{[1
]}

机构：

[1] BRISTOL MYERS SQUIBB CO,PHARMACEUT RES INST,DEPT ANALYT RES & DEV,PRINCETON,NJ 08543

来源：

JOURNAL OF NATURAL PRODUCTS | 1994年 / 57卷 / 10期

关键词：

D O I：

10.1021/np50112a008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Analytical methodology developed for the trace analysis of natural products in crude extracts was utilized for the rapid and systematic structure elucidation of taxanes in Taxus extracts and process intermediates. This method integrates analytical hplc, uv detection, uv spectroscopy, full-scan ionspray mass spectrometry and tandem mass spectrometry on-line. The identification of structure of a taxane is based on comparing the mass spectrometric characteristics of the taxane with the paclitaxel substructural ''template.'' Analytical data for taxanes in preparations from Taxus brevifolia and Taxus baccata were observed, including chromatographic characteristics using a standard hplc system, molecular weight, and collision induced dissociation (cid) tandem mass spectrometry (ms/ms) product ion spectra. The data obtained for 18 taxanes from natural sources using this method provided a taxane profile database useful for the rapid identification of taxanes in mixtures and samples of limited quantity.

引用

页码：1391 / 1403

页数：13

共 26 条

[1] DETERMINATION OF TAXOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY [J].

AURIOLA, SOK ;

LEPISTO, AM ;

NAARANLAHTI, T ;

LAPINJOKI, SP .

JOURNAL OF CHROMATOGRAPHY, 1992, 594 (1-2) :153-158

[2] THE CHEMISTRY AND OCCURRENCE OF TAXANE DERIVATIVES .5. TAXANES FROM THE NEEDLES OF TAXUS-WALLICHIANA [J].

BARBONI, L ;

GARIBOLDI, P ;

TORREGIANI, E ;

APPENDINO, G ;

GABETTA, B ;

ZINI, G ;

BOMBARDELLI, E .

PHYTOCHEMISTRY, 1993, 33 (01) :145-150

[3] ANALYSIS OF TAXOL AND RELATED DITERPENOIDS FROM CELL-CULTURES BY LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY [J].

BITSCH, F ;

MA, WW ;

MACDONALD, F ;

NIEDER, M ;

SHACKLETON, CHL .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 615 (02) :273-280

[4] TAXOID SIDE-CHAIN STRUCTURE DETERMINATION BY ELECTROSPRAY-IONIZATION TANDEM MASS-SPECTROMETRY [J].

BITSCH, F ;

SHACKLETON, CHL ;

MA, WW ;

PARK, G ;

NIEDER, M .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (10) :891-894

[5] ANALYSIS OF TAXOL AND RELATED TAXANES FROM TAXUS-CANADENSIS USING LIQUID-CHROMATOGRAPHY COMBINED WITH MASS-SPECTROMETRY OR TANDEM MASS-SPECTROMETRY [J].

BLAY, PKS ;

THIBAULT, P ;

THIBERGE, N ;

KIECKEN, B ;

LEBRUN, A ;

MERCURE, C .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (07) :626-634

[6]

BLECHERT S, 1990, ALKALOIDS CHEM PHARM, V39, P195

[7] ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY [J].

BRUINS, AP ;

COVEY, TR ;

HENION, JD .

ANALYTICAL CHEMISTRY, 1987, 59 (22) :2642-2646

[8]

Busch KL., 1988, MASS SPECTROMETRY MA

[9] THE PHOTOCHEMISTRY OF TAXOL - SYNTHESIS OF A NOVEL PENTACYCLIC TAXOL ISOMER [J].

CHEN, SH ;

COMBS, CM ;

HILL, SE ;

FARINA, V ;

DOYLE, TW .

TETRAHEDRON LETTERS, 1992, 33 (50) :7679-7680

[10]

Della Casa de Marcano D. P., 1975, J CHEM SOC CHEM COMM, V9, P365

← 1 2 3 →