THE MAIZE TRANSPOSABLE ELEMENT SYSTEM AC DS AS A MUTAGEN IN ARABIDOPSIS - IDENTIFICATION OF AN ALBINO MUTATION INDUCED BY DS INSERTION

被引:107
作者
LONG, D [1 ]
MARTIN, M [1 ]
SUNDBERG, E [1 ]
SWINBURNE, J [1 ]
PUANGSOMLEE, P [1 ]
COUPLAND, G [1 ]
机构
[1] JOHN INNES CTR,CAMBRIDGE LAB,DEPT MOLEC GENET,COLNEY LANE,NORWICH NR4 7UJ,ENGLAND
关键词
D O I
10.1073/pnas.90.21.10370
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A two-component transposon system based on the Ac element of maize was used as a mutagen in Arabidopsis thaliana. Transposition of a Ds element marked with a hygromycin-resistance gene was activated from four different locations in the Arabidopsis genome. The progeny of 201 plants carrying independent transposition events were screened for mutants with severe, visible phenotypes. Seven mutants were identified and four of them were analyzed genetically. Three mutations were shown to be very closely linked to a transposed copy of the element. Moreover, a mutation (alb3) causing an albino phenotype was conclusively shown to be caused by insertion of the Ds element: somatic and germinal reversion of the mutation occurred in the presence of the transposase gene but not in its absence, and in three revertants the Ds had excised from its position in the mutant line. The DNA adjacent to Ds in the mutant was isolated and it was demonstrated that revertants retained part of the 8-bp duplication caused by insertion of Ds. These experiments indicate that the Ac/Ds system can be used as an insertional mutagen in the heterologous host Arabidopsis, which will permit the isolation of genes from this species by transposon tagging.
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页码:10370 / 10374
页数:5
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