DETERMINATION OF EXEMESTANE, A NEW AROMATASE INHIBITOR, IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

被引：16

作者：

BREDA, M ^{[1
]}

PIANEZZOLA, E ^{[1
]}

BENEDETTI, MS ^{[1
]}

机构：

[1] FARMITALIA CARLO ERBA SPA,DEPT PHARMACOKINET & METAB,R&D,VIA PAPA GIOVANNI XXIII,I-20014 NERVIANO,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 620卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80008-R

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and selective high-performance liquid chromatographic method for the determination of 6-methylen-androsta-1,4-diene-3,17-dione (exemestane) and its 17-dihydro metabolite in human plasma has been developed. The analytes and internal standard (Norgestrel) were extracted from plasma samples with a methylene chloride-iso-octane mixture; the organic phase was dried and the residue was reconstituted with an acetonitrile-water mixture, then analyzed by reversed-phase liquid chromatography. Quantification was achieved by ultraviolet detection of the eluate. The linearity, precision and accuracy of the method were evaluated. No interference from the constituents of human blank plasma was observed. The lower limit of quantification was 10 ng/ml plasma. The suitability of the method for in vivo samples was checked by analysis of plasma samples drawn from healthy male volunteers who had received a 200-mg single oral dose of the test compound.

引用

页码：225 / 231

页数：7

共 16 条

[1] INACTIVATION OF AROMATASE INVITRO BY 4-HYDROXY-4-ANDROSTENE-3, 17-DIONE AND 4-ACETOXY-4-ANDROSTENE-3, 17-DIONE AND SUSTAINED EFFECTS INVIVO [J].