MINI-CHROMOSOME FROM BK VIRUS AS A TEMPLATE FOR TRANSCRIPTION INVITRO

被引：29

作者：

MENEGUZZI, G

PIGNATTI, PF

BARBANTIBRODANO, G

MILANESI, G

机构：

[1] CNR,IST GENET,GENET BIOCHIM & EVOLUZ LAB,PAVIA,ITALY

[2] UNIV FERRARA,IST MICROBIOL,I-44100 FERRARA,ITALY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1978年 / 75卷 / 03期

关键词：

D O I：

10.1073/pnas.75.3.1126

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

BK virus DNA can be extracted from virions as a nucleoprotein complex containing about 20 nucleosomes. Transcription of this 'minichromosome' with Escherichia coli RNA polymerase indicates that both initiation and elongation of RNA chains are reduced by the presence of nucleosomes. Hybridization analysis of RNA made on the complex shows preferential transcription of one region of BK virus genome. No increase in strand selection is observed with respect to transcription of purified superhelical BK virus DNA.

引用

页码：1126 / 1130

页数：5

共 39 条

[11] GARDNER SD, 1971, LANCET, V1, P1253
[12] FOLDING OF DNA DOUBLE HELIX IN CHROMATIN-LIKE STRUCTURES FROM SIMIAN VIRUS 40
GERMOND, JE
HIRT, B
OUDET, P
GROSSBELLARD, M
CHAMBON, P
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (05) : 1843 - 1847
[13] PROPERTIES OF NUCLEOPROTEIN COMPLEXES CONTAINING REPLICATING POLYOMADNA
GOLDSTEIN, DA
HALL, MR
MEINKE, W
[J]. JOURNAL OF VIROLOGY, 1973, 12 (04) : 887 - 900
[14] GREEN M H, 1975, INSERM (Institut National de la Sante et de la Recherche Medicale) Colloque, V47, P33
[15] CHROMATIN STRUCTURE - DEDUCED FROM A MINICHROMOSOME
GRIFFITH, JD
[J]. SCIENCE, 1975, 187 (4182) : 1202 - 1203
[16] TRANSCRIPTION AND REISOLATION OF SIMIAN-VIRUS 40 NUCLEOPROTEIN COMPLEX
HALL, MR
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 76 (03) : 698 - 704
[17] TEMPLATE ACTIVITIES OF PHIX-174 REPLICATIVE ALLOMORPHIC DEOXYRIBONUCLEIC ACIDS
HAYASHI, Y
HAYASHI, M
[J]. BIOCHEMISTRY, 1971, 10 (23) : 4212 - &
[18] CHARACTERIZATION OF HUMAN PAPOVAVIRUS BK DNA
HOWLEY, PM
MULLARKEY, MF
TAKEMOTO, KK
MARTIN, MA
[J]. JOURNAL OF VIROLOGY, 1975, 15 (01) : 173 - 181
[19] PHYSICAL MAP OF BK VIRUS GENOME
HOWLEY, PM
KHOURY, G
BYRNE, JC
TAKEMOTO, KK
MARTIN, MA
[J]. JOURNAL OF VIROLOGY, 1975, 16 (04) : 959 - 973
[20] STRUCTURE AND FUNCTION OF POLYPEPTIDES IN SIMIAN VIRUS 40 .1. EXISTENCE OF SUBVIRAL DEOXYNUCLEOPROTEIN COMPLEXES
HUANG, ES
ESTES, MK
PAGANO, JS
[J]. JOURNAL OF VIROLOGY, 1972, 9 (06) : 923 - &

← 1 2 3 4 →