MINI-CHROMOSOME FROM BK VIRUS AS A TEMPLATE FOR TRANSCRIPTION INVITRO

被引：29

作者：

MENEGUZZI, G

PIGNATTI, PF

BARBANTIBRODANO, G

MILANESI, G

机构：

[1] CNR,IST GENET,GENET BIOCHIM & EVOLUZ LAB,PAVIA,ITALY

[2] UNIV FERRARA,IST MICROBIOL,I-44100 FERRARA,ITALY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1978年 / 75卷 / 03期

关键词：

D O I：

10.1073/pnas.75.3.1126

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

BK virus DNA can be extracted from virions as a nucleoprotein complex containing about 20 nucleosomes. Transcription of this 'minichromosome' with Escherichia coli RNA polymerase indicates that both initiation and elongation of RNA chains are reduced by the presence of nucleosomes. Hybridization analysis of RNA made on the complex shows preferential transcription of one region of BK virus genome. No increase in strand selection is observed with respect to transcription of purified superhelical BK virus DNA.

引用

页码：1126 / 1130

页数：5

共 39 条

[31] PREVALENCE IN ITALY OF ANTIBODIES TO A NEW HUMAN PAPOVAVIRUS (BK VIRUS) [J].