REGULATION OF THE YEAST DNA-REPLICATION GENES THROUGH THE MLU-I CELL-CYCLE BOX IS DEPENDENT ON SWI6

被引：42

作者：

VERMA, R ^{[1
]}

SMILEY, J ^{[1
]}

ANDREWS, B ^{[1
]}

CAMPBELL, JL ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MOLEC & MED GENET,TORONTO M5S 1A8,ONTARIO,CANADA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 20期

关键词：

D O I：

10.1073/pnas.89.20.9479

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In Saccharomyces cerevisiae, at least 17 DNA replication genes are coordinately expressed at the G1/S boundary during the cell cycle. All of these genes have the DNA sequence element ACGCGT in their 5' upstream regulatory regions. This sequence has been shown to be essential for periodic expression of the POL1, CDC9, and TMP1 genes. The cyclin (CLN1 and CLN2) and HO genes are another subset of genes that are expressed with the same timing as the DNA replication genes. Their periodic expression requires the participation of two well-characterized transcriptional activators: the SWI4 and SWI6 gene products. In this study, we present evidence that SWI6 contributes to the regulation of DNA replication genes as well. Surprisingly, a preferential requirement for SWI6 over SWI4 is observed in our studies of ACGCGT-dependent reporter gene expression in vivo. This selectivity has not been observed for the other G1/S genes. Correlating with the in vivo results, protein-DNA complexes formed in vitro on multimeric ACGCGT elements are either abolished or reduced in swi6DELTA deletion mutants.

引用

页码：9479 / 9483

页数：5

共 26 条

[11] ISOLATION OF THE GENE ENCODING YEAST DNA-POLYMERASE-I [J].

JOHNSON, LM ;

SNYDER, M ;

CHANG, LMS ;

DAVIS, RW ;

CAMPBELL, JL .

CELL, 1985, 43 (01) :369-377

[12] A NEGATIVE REGULATOR OF HO-TRANSCRIPTION, SIN1 (SPT2), IS A NONSPECIFIC DNA-BINDING PROTEIN RELATED TO HMG1 [J].

KRUGER, W ;

HERSKOWITZ, I .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (08) :4135-4146

[13] SWI6 PROTEIN IS REQUIRED FOR TRANSCRIPTION OF THE PERIODICALLY EXPRESSED DNA-SYNTHESIS GENES IN BUDDING YEAST [J].

LOWNDES, NF ;

JOHNSON, AL ;

BREEDEN, L ;

JOHNSTON, LH .

NATURE, 1992, 357 (6378) :505-508

[14] CONTROL OF DNA-SYNTHESIS GENES IN FISSION YEAST BY THE CELL-CYCLE GENE CDC10+ [J].

LOWNDES, NF ;

MCINERNY, CJ ;

JOHNSON, AL ;

FANTES, PA ;

JOHNSTON, LH .

NATURE, 1992, 355 (6359) :449-453

[15] COORDINATION OF EXPRESSION OF DNA-SYNTHESIS GENES IN BUDDING YEAST BY A CELL-CYCLE REGULATED TRANS FACTOR [J].

LOWNDES, NF ;

JOHNSON, AL ;

JOHNSTON, LH .

NATURE, 1991, 350 (6315) :247-250

[16] DIRECT INDUCTION OF G1-SPECIFIC TRANSCRIPTS FOLLOWING REACTIVATION OF THE CDC28 KINASE IN THE ABSENCE OF DENOVO PROTEIN-SYNTHESIS [J].

MARINI, NJ ;

REED, SI .

GENES & DEVELOPMENT, 1992, 6 (04) :557-567

[17] TRANSCRIPTIONAL REGULATION OF THE CELL CYCLE-DEPENDENT THYMIDYLATE SYNTHASE GENE OF SACCHAROMYCES-CEREVISIAE [J].

MCINTOSH, EM ;

ORD, RW ;

STORMS, RK .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (11) :4616-4624

[18] CHARACTERIZATION OF A SHORT, CIS-ACTING DNA-SEQUENCE WHICH CONVEYS CELL-CYCLE STAGE-DEPENDENT TRANSCRIPTION IN SACCHAROMYCES-CEREVISIAE [J].

MCINTOSH, EM ;

ATKINSON, T ;

STORMS, RK ;

SMITH, M .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (01) :329-337

[19]

Miller J.H., 1972, EXPT MOL GENETICS, P1

[20] A REPETITIVE DNA-SEQUENCE THAT CONFERS CELL-CYCLE START (CDC28)-DEPENDENT TRANSCRIPTION OF THE HO GENE IN YEAST [J].

NASMYTH, K .

CELL, 1985, 42 (01) :225-235

← 1 2 3 →