RELATIONSHIP BETWEEN ETHANOL-CONSUMPTION RATE AND PROCHIRAL KETONE REDUCTION RATE IN BAKERS-YEAST

被引：15

作者：

KOMETANI, T ^{[1
]}

MORITA, Y ^{[1
]}

KIYAMA, Y ^{[1
]}

YOSHI, H ^{[1
]}

MATSUNO, R ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,DEPT FOOD SCI & TECHNOL,KYOTO 606,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 80卷 / 02期

关键词：

BAKERS YEAST; ASYMMETRIC REDUCTION; ETHANOL; K(L)A;

D O I：

10.1016/0922-338X(95)93222-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The relationship between ethanol consumption and the bioreduction of prochiral ketones in bakers' yeast cells using ethanol as a reductant for NAD(P)H regeneration was examined and the efficiency (nu(sub)/nu(EtOH)) correlated to k(L) alpha. Ethanol was consumed at almost the same specific rate in the presence or absence of the ketones. In the reduction of ethyl acetoacetate catalyzed by NADPH-dependent oxidoreductase, the efficiency (nu(EA)/nu(ELOH)) decreased with increasing k(L) alpha and was about 0.5 at the actual operating condition of kLa of 100 h(-1). The reduction of acetol catalyzed by NADH-dependent oxidoreductase proceeded through the use of a portion of NADH in the regenerated NADH, and the efficiency (nu(acetot)/nu(EtOH)) had a maximum (1.9) at k(L) alpha of 20h(-1)

引用

页码：208 / 210

页数：3

共 7 条

[1] BAKERS-YEAST MEDIATED BIOREDUCTION - PRACTICAL PROCEDURE USING ETOH AS ENERGY-SOURCE [J].

KOMETANI, T ;

KITATSUJI, E ;

MATSUNO, R .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1991, 71 (03) :197-199

[2] OXIDATIVE RESOLUTION WITH BAKERS-YEAST FOR LARGE-SCALE PRODUCTION OF CHIRAL 1,2-ALKANEDIOLS [J].

KOMETANI, T ;

MORITA, Y ;

YOSHII, H ;

KIYAMA, Y ;

MATSUNO, R .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1995, 80 (02) :180-184

[3] BIOREDUCTION OF KETONES MEDIATED BY BAKERS-YEAST WITH ACETATE AS ULTIMATE REDUCING AGENT [J].

KOMETANI, T ;

KITATSUJI, E ;

MATSUNO, R .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1991, 55 (03) :867-868

[4] LARGE-SCALE PREPARATION OF (S)-ETHYL 3-HYDROXYBUTANOATE WITH A HIGH ENANTIOMERIC EXCESS THROUGH BAKERS YEAST-MEDIATED BIOREDUCTION [J].

KOMETANI, T ;

YOSHII, H ;

KITATSUJI, E ;

NISHIMURA, H ;

MATSUNO, R .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1993, 76 (01) :33-37

[5] NAD(P)H REGENERATION USING ETHANOL AS AN ENERGY-SOURCE IN BAKERS YEAST-MEDIATED BIOREDUCTION [J].

KOMETANI, T ;

MORITA, Y ;

FURUI, H ;

YOSHII, H ;

MATSUNO, R .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1994, 77 (01) :13-16

[6] BAKERS-YEAST MEDIATED BIOREDUCTION - A NEW PROCEDURE USING ETHANOL AS AN ENERGY-SOURCE [J].

KOMETANI, T ;

KITATSUJI, E ;

MATSUNO, R .

CHEMISTRY LETTERS, 1989, (08) :1465-1466

[7] LARGE-SCALE PREPARATION OF (R)-1,2-PROPANEDIOL THROUGH BAKER YEAST-MEDIATED BIOREDUCTION [J].

KOMETANI, T ;

YOSHII, H ;

TAKEUCHI, Y ;

MATSUNO, R .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1993, 76 (05) :414-415

← 1 →