INDUCTION OF CHROMOSOMAL-ABERRATIONS WITH BENZON NUCLEASE IN CHINESE-HAMSTER OVARY (CHO) CELLS

被引：4

作者：

JOHANNES, C ^{[1
]}

OBE, G ^{[1
]}

机构：

[1] UNIV ESSEN GESAMTHSCH,DEPT GENET,D-45117 ESSEN,GERMANY

来源：

MUTATION RESEARCH LETTERS | 1994年 / 325卷 / 2-3期

关键词：

BENZON NUCLEASE; CHROMOSOME ABERRATION INDUCTION; CHO CELLS;

D O I：

10.1016/0165-7992(94)90010-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Benzon nuclease, an endonuclease originating from Serratia marcescens, was tested for its chromosome breaking activity in Chinese hamster ovary cells. Using a permeabilizing method with hypertonic glycerol, benzon nuclease induced chromosomal aberrations in an S-phase independent manner. The frequencies of polycentric chromosomes were correlated with the dose of the enzyme and the intercellular distribution of aberrations was overdispersed.

引用

页码：113 / 116

页数：4

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