KINETIC-PROPERTIES OF SOLUBLE AND IMMOBILIZED CANDIDA-RUGOSA LIPASE

被引：7

作者：

VIRTO, MD

AGUD, I

MONTERO, S

BLANCO, A

SOLOZABAL, R

LASCARAY, JM

LLAMA, MJ

SERRA, JL

LANDETA, LC

DERENOBALES, M

机构：

[1] INASMET, DEPT BIOTECHNOL, SAN SEBASTIAN, SPAIN

[2] UNIV BASQUE COUNTRY, FAC FARM, DEPT BIOQUIM & BIOL MOLEC, E-01080 VITORIA, SPAIN

[3] UNIV BASQUE COUNTRY, FAC CIENCIAS, DEPT BIOQUIM & BIOL MOLEC, E-48080 BILBAO, SPAIN

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 50卷 / 02期

关键词：

LIPASE; EC; 3.1.1.3; IMMOBILIZED ENZYME; ANIMAL FATS HYDROLYSIS; ORGANIC SOLVENTS; CANDIDA RUGOSA; KINETIC PARAMETERS;

D O I：

10.1007/BF02783449

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilized lipase (triacylglycerol ester hydrolase, EC 3.1.1.3) from Candida rugosa has been immobilized on commercially available microporous polypropylene and used for the batch hydrolysis of different animal fats. The effect of the reaction products at concentrations similar to those obtained at 90% hydrolysis, both on soluble and immobilized lipase, was studied. Glycerol showed low inhibitory effect but oleic acid caused 50% inhibition. A mixture of free fatty acids present in the complete hydrolysis of beef tallow inhibited lipase activity more than 70%. The stability of the enzyme (both soluble and immobilized) was highest in the presence of 20% isooctane. The apparent Michaelis constant for each substrate for the soluble enzyme did not change on immobilization.

引用

页码：127 / 136

页数：10

共 14 条

[1] POSITIONAL AND CHAIN SPECIFICITIES OF CANDIDA-CYLINDRACEA LIPASE [J].

BENZONANA, G ;

ESPOSITO, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 231 (01) :15-+

[2] ETUDE CINETIQUE DE LACTION DE LA LIPASE PANCREATIQUE SUR DES TRIGLYCERIDES EN EMULSION ESSAI DUNE ENZYMOLOGIE EN MILIEU HETEROGENE [J].

BENZONANA, G ;

DESNUELLE, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 105 (01) :121-+

[3] HYDROLYSIS OF ANIMAL FATS BY LIPASE AT TEMPERATURES BELOW THEIR MELTING-POINTS [J].

DERENOBALES, M ;

AGUD, I ;

LASCARAY, JM ;

MUGICA, JC ;

LANDETA, LC ;

SOLOZABAL, R .

BIOTECHNOLOGY LETTERS, 1992, 14 (08) :683-688

[4] LIPASE KINETICS - HYDROLYSIS OF TRIACETIN BY LIPASE FROM CANDIDA-CYLINDRACEA IN A HOLLOW-FIBER MEMBRANE REACTOR [J].

GUIT, RPM ;

KLOOSTERMAN, M ;

MEINDERSMA, GW ;

MAYER, M ;

MEIJER, EM .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (07) :727-732

[5] LIPASE-CATALYZED HYDROLYSIS OF PALM OIL [J].

KHOR, HT ;

TAN, NH ;

CHUA, CL .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1986, 63 (04) :538-540

[6] LIPASE KINETICS - ONLINE MEASUREMENT OF THE INTERFACIAL AREA OF EMULSIONS [J].

KIERKELS, JGT ;

VLEUGELS, LFW ;

KERN, JHA ;

MEIJER, EM ;

KLOOSTERMAN, M .

ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (10) :760-763

[7]

KIM KH, 1984, LIPIDS, V19, P975, DOI 10.1007/BF02534737

[8]

MARTINEZ O, 1992, J CHEM TECHNOL BIOT, V53, P373, DOI 10.1002/jctb.280530409

[9] THE LARGE-N MAGNETIZATION IN MATRIX MODELS REVISITED [J].

MONTALDI, E ;

MOLINARI, L .

NUOVO CIMENTO DELLA SOCIETA ITALIANA DI FISICA D-CONDENSED MATTER ATOMIC MOLECULAR AND CHEMICAL PHYSICS FLUIDS PLASMAS BIOPHYSICS, 1993, 15 (2-3) :293-298

[10]

MUKATAKA S, 1985, J FERMENT TECHNOL, V63, P461

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