COMPLEX PATTERNS OF TRANSCRIPTION OF A DROSOPHILA RETROTRANSPOSON IN-VIVO AND IN-VITRO BY RNA-POLYMERASE-II AND RNA-POLYMERASE-III

被引：6

作者：

ARKHIPOVA, IR ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,ENGELHARDT INST MOLEC BIOL,MOSCOW,RUSSIA

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 21期

关键词：

D O I：

10.1093/nar/23.21.4480

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mdg1 retrovirus-like retrotransposon of Drosophila melanogaster was found to possess a complex promoter which can be transcribed by both RNA polymerases II and III (pol II and pol III), pol III transcription, which is not typical of protein-coding genes, is driven by the sequences located in the long terminal repeat (LTR) of mdg1, predominantly within the transcribed region and is initiated 10 bp upstream from the regular pol II RNA start site, The pol III RNA start site is observed not only in in vitro transcription reactions, but also in total RNA isolated from tissue culture cells, larvae, pupae and adult flies, A possible role of pol III transcription in mechanisms controlling the expression of full-length mdg1-encoded transcripts in the developing fly, which are apparently relaxed in cell culture, is discussed.

引用

页码：4480 / 4487

页数：8

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