STRUCTURE OF THE HUMAN GASTRIC H,K-ATPASE GENE AND COMPARISON OF THE 5'-FLANKING SEQUENCES OF THE HUMAN AND RAT GENES

被引:39
作者
NEWMAN, PR [1 ]
GREEB, J [1 ]
KEETON, TP [1 ]
REYES, AA [1 ]
SHULL, GE [1 ]
机构
[1] UNIV CINCINNATI, DEPT MOLEC GENET BIOCHEM & MICROBIOL, 231 BETHESDA AVE, CINCINNATI, OH 45267 USA
关键词
D O I
10.1089/dna.1990.9.749
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated and analyzed the genes encoding the human and rat gastric H,K-ATPase catalytic subunits. The complete sequence of the human gene, including 2.2 kb of 5'-flanking sequence, and the 5' end of the rat gene, including exons 1-4 and 2.5 kb of 5'-flanking sequence, have been determined. The human gene contains 22 exons. Its intron-exon organization is identical to that of the Na,K-ATPase gene, except that exon 6 corresponds to a fusion of exons 6 and 7 of the Na,K-ATPase gene. The transcription initiation sites of both the human and rat genes were determined by primer extension and S1 nuclease protection analyses. Comparison of the 5'-flanking regions of the human and rat genes revealed three extended regions of high sequence similarity, one of which includes a potential TATA box and other basic promoter elements beginning about 30 nucleotides upstream of the transcription start site. Other conserved sequences, including possible response elements for Ca2+ and cAMP, which are known intracellular mediators of acid secretion, are located up to 2 kb 5' to the transcription initiation site.
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收藏
页码:749 / 762
页数:14
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