A SYNTHETIC PROCEDURE FOR THE PREPARATION OF OLIGONUCLEOTIDES WITHOUT USING AMMONIA AND ITS APPLICATION FOR THE SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING O-4-ALKYL THYMIDINES

被引：27

作者：

ERITJA, R ^{[1
]}

ROBLES, J ^{[1
]}

AVINO, A ^{[1
]}

ALBERICIO, F ^{[1
]}

PEDROSO, E ^{[1
]}

机构：

[1] UNIV BARCELONA,DEPT ORGAN CHEM,E-08028 BARCELONA,SPAIN

来源：

TETRAHEDRON | 1992年 / 48卷 / 20期

关键词：

D O I：

10.1016/S0040-4020(01)92195-7

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The preparation of 5'O-dimethoxytrityl (DMT) and p-nitrophenylethyl (NPEOC, NPE) protected nucleosides linked to 4-(2-hydroxyethyl)-3-nitrobenzoic acid derivatives is described. These products attached to controlled-pore glass supports and together with DMT and NPE-protected nucleoside cyanoethyl phosphoramidites allows for the first time the preparation of short oligonucleotides containing the ammonia sensitive mutagenic bases O-4-propyl and O-4-butyl thymidines.

引用

页码：4171 / 4182

页数：12

共 23 条

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