High-speed centrifugation induces aggregation of extracellular vesicles

被引：438

作者：

Linares, Romain ^{[1
]}

Tan, Sisareuth ^{[1
]}

Gounou, Celine ^{[1
]}

Arraud, Nicolas ^{[1
]}

Brisson, Alain R. ^{[1
,2
]}

机构：

[1] Univ Bordeaux, Mol Imaging & NanoBioTechnol, Pessac, France

[2] Inst Univ France, Paris, France

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2015年 / 4卷

关键词：

extracellular vesicles; blood plasma; cryo-electron microscopy; immuno-gold electron microscopy; flow cytometry; vesicle aggregation; centrifugation;

D O I：

10.3402/jev.v4.29509

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EV aggregates composed of a mixture of EVs of various phenotypes and morphologies. The presence of aggregates made of EVs of different phenotypes may lead to erroneous interpretation concerning the existence of EVs harbouring surface antigens from different cell origins.

引用

收藏

页数：7

相关论文

共 41 条

[1]

Antigenic composition of single nano-sized extracellular blood vesicles [J].

Arakelyan, Anush ;

Ivanova, Oxana ;

Vasilieva, Elena ;

Grivel, Jean-Charles ;

Margolis, Leonid .

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2015, 11 (03) :489-498

[2]

A simple flow cytometry method improves the detection of phosphatidylserine-exposing extracellular vesicles [J].

Arraud, N. ;

Gounou, C. ;

Linares, R. ;

Brisson, A. R. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2015, 13 (02) :237-247

[3]

Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration [J].

Arraud, N. ;

Linares, R. ;

Tan, S. ;

Gounou, C. ;

Pasquet, J. -M. ;

Mornet, S. ;

Brisson, A. R. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2014, 12 (05) :614-627

[4]

Arraud N, 2015, CYTOMETRY A

[5]

Single-step isolation of extracellular vesicles by size-exclusion chromatography [J].

Boing, Anita N. ;

van der Pol, Edwin ;

Grootemaat, Anita E. ;

Coumans, Frank A. W. ;

Sturk, Auguste ;

Nieuwland, Rienk .

JOURNAL OF EXTRACELLULAR VESICLES, 2014, 3 (01)

[6]

A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer [J].

Chandler, W. L. ;

Yeung, W. ;

Tait, J. F. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2011, 9 (06) :1216-1224

[7]

Lactadherin and clearance of platelet-derived microvesicles [J].

Dasgupta, Swapan K. ;

Abdel-Monem, Hanan ;

Niravath, Polly ;

Le, Anhquyen ;

Bellera, Ricardo V. ;

Langlois, Kimberly ;

Nagata, Shigekazu ;

Rumbaut, Rolando E. ;

Thiagarajan, Perumal .

BLOOD, 2009, 113 (06) :1332-1339

[8]

Elevated numbers of tissue-factor exposing microparticles correlate with components of the metabolic syndrome in uncomplicated type 2 diabetes mellitus [J].

Diamant, M ;

Nieuwland, R ;

Pablo, RF ;

Sturk, A ;

Smit, JWA ;

Radder, JK .

CIRCULATION, 2002, 106 (19) :2442-2447

[9]

Imaging Flow Cytometry Elucidates Limitations of Microparticle Analysis by Conventional Flow Cytometry [J].

Erdbruegger, Uta ;

Rudy, Christine K. ;

Etter, Mark E. ;

Dryden, Kelly A. ;

Yeager, Mark ;

Klibanov, Alexander L. ;

Lannigan, Joanne .

CYTOMETRY PART A, 2014, 85A (09) :756-770

[10]

As we wait: coping with an imperfect nomenclature for extracellular vesicles [J].

Gould, Stephen J. ;

Raposo, Graca .

JOURNAL OF EXTRACELLULAR VESICLES, 2013, 2 (01)

← 1 2 3 4 5 →