THE SEPARATION OF PECTINYLASE FROM BETA-GLUCOSIDASE IN A COMMERCIAL PREPARATION

被引:23
作者
MARTINO, A
PIFFERI, PG
SPAGNA, G
机构
[1] Department of Industrial Chemistry and Materials, University of Bologna, Bologna, 40136
关键词
SEPARATION; PURIFICATION; ENZYMES; PECTINYLASE; BETA-GLUCOSIDASE;
D O I
10.1002/jctb.280610313
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This paper describes a new, simple and inexpensive procedure for separating pectinlyase (PL, EC 4.2.2.3) from beta-glucosidase (beta-glu, EC 3.2.1.21), both of which have potential for use in the beverage processing industry. The method described here, which entails the treatment of crude preparations with bentonite (4% (w/v)) and the acidification of the resulting supernatant to pH 3.5, leads to the production of two enzymic solutions which contain PL and beta-glu, respectively. In both solutions the amount of brown pigment is considerably less than in the crude mixture, and partial purification from extraneous proteins is also achieved.
引用
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页码:255 / 260
页数:6
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