THE SEPARATION OF PECTINYLASE FROM BETA-GLUCOSIDASE IN A COMMERCIAL PREPARATION

This paper describes a new, simple and inexpensive procedure for separating pectinlyase (PL, EC 4.2.2.3) from beta-glucosidase (beta-glu, EC 3.2.1.21), both of which have potential for use in the beverage processing industry. The method described here, which entails the treatment of crude preparations with bentonite (4% (w/v)) and the acidification of the resulting supernatant to pH 3.5, leads to the production of two enzymic solutions which contain PL and beta-glu, respectively. In both solutions the amount of brown pigment is considerably less than in the crude mixture, and partial purification from extraneous proteins is also achieved.