EFFECTS OF LIPOPOLYSACCHARIDES AND PROSTAGLANDINS ON RAT BONE CELL CALCIUM AND CYCLIC-AMP

Bone cells were isolated by collagenase digestion of 19-21 day old fetal rat calvaria. At 37 °C, lipopolysaccharides decreased Ca uptake 30-70 per cent below control values. At 4 °C, 100-900 per cent increases in Ca uptake were observed. Incubation with lipid A produced similar effects. No effects of lipopolysaccharides on cyclic AMP were noted. Prostaglandins E1, E2 and A1 had no effects on calcium transport but produced characteristic increases in cyclic AMP. © 1979.