Misfit dislocations in [001] GaAs/In0.25Ga0.75As/GaAs multiquantum well heterostructures are studied by transmission electron microscopy. Two sets of misfit dislocation networks have been observed; one is a set of conventional 60 degrees misfit dislocations lying along <110>, and the other is a set of edge dislocations lying along <100>. The location of these misfit dislocations with respect to the strained layers is investigated. A qualitative explanation for the generation mechanism of the misfit dislocations lying along <100> is given.