A RAPID METHOD FOR SEPARATION OF ANOMERIC SACCHARIDES USING A CYCLODEXTRIN-BONDED PHASE AND FOR INVESTIGATION OF MUTAROTATION

被引：22

作者：

SCHUMACHER, D ^{[1
]}

KROH, LW ^{[1
]}

机构：

[1] TECH UNIV BERLIN,INST FOOD CHEM,D-13355 BERLIN,GERMANY

来源：

FOOD CHEMISTRY | 1995年 / 54卷 / 04期

关键词：

D O I：

10.1016/0308-8146(95)00059-R

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

HPLC separation of anomeric sugars using a beta-cyclodextrin bonded phase and an eluent ethyl acetate/methanol/water has been achieved. HPLC is a useful method for analytically separating the anomers of sugars in a mutarotated system and is a good supplement to the nuclear magnetic resonance. By means of this technique investigations of the mutarotation of D-glucose gave 36% alpha-form and 64% beta-form. The pH dependence of mutarotation in an organic solvent was examined and discussed. Further mono- and disaccharides were separated in anomers. Furanoid forms of the sugar can also be determined by HPLC.

引用

页码：353 / 356

页数：4

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