A URIDINE-RICH SEQUENCE REQUIRED FOR TRANSLATION OF PROKARYOTIC MESSENGER-RNA

被引:62
作者
ZHANG, JR [1 ]
DEUTSCHER, MP [1 ]
机构
[1] UNIV CONNECTICUT, CTR HLTH, DEPT BIOCHEM, FARMINGTON, CT 06030 USA
关键词
SHINE-DALGARNO SEQUENCE; RIBOSOMAL PROTEIN S1; PROTEIN SYNTHESIS;
D O I
10.1073/pnas.89.7.2605
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Binding of 30S ribosomal subunits to mRNA during the initiation of prokaryotic translation is known to be influenced by the initiation codon and the Shine-Dalgarno sequence. Site-directed mutagenesis of rnd, the Escherichia coli gene encoding RNase D, has now shown that a U8 sequence upstream of the Shine-Dalgarno region is also essential for expression of this mRNA. Alteration of two to five uridine residues within this sequence has no effect on mRNA levels but decreases RNase D protein and activity by as much as 95%, indicating that the U-rich sequence acts as an enhancer of translation. Moreover, mutant transcripts bind to 30S ribosomes in vitro with lower affinity than their wild-type counterparts, suggesting that the role of the U8 sequence is in the initial binding of ribosomes to the translation initiation region of the message. These data demonstrate that sequences other than those previously recognized can be essential for translation initiation.
引用
收藏
页码:2605 / 2609
页数:5
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