MICROTUBULE-ASSOCIATED PROTEIN-TAU IS HYPERPHOSPHORYLATED DURING MITOSIS IN THE HUMAN NEUROBLASTOMA CELL-LINE SH-SY5Y

被引:101
作者
POPE, WB [1 ]
LAMBERT, MP [1 ]
LEYPOLD, B [1 ]
SEUPAUL, R [1 ]
SLETTEN, L [1 ]
KRAFFT, G [1 ]
KLEIN, WL [1 ]
机构
[1] ABBOTT LABS,ABBOTT PK,IL 60064
关键词
D O I
10.1006/exnr.1994.1057
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A phosphorylated tau epitope specific for paired helical filaments in Alzheimer's disease is recognized by monoclonal antibody PHF-1. Healthy adult brains lack the PHF-1 epitope (PHF-1 tau), but it is transiently expressed by immature neurons during development. We have found that proliferating SH-SY5Y human neuroblastoma cells also express PHF-1 tau. Consistent with the recent finding that cell-cycle-dependent kinases can phosphorylate tau in vitro, how cytometry showed that mitotic SH-SY5Y cells were up to 18-fold more PHF-1 immunoreactive than nonmitotic cells. On immunoblots, PHF-1 tau in mitotic and nonmitotic cells also was strikingly different. First, mitosis induced a prominent PHF-1 reactive band at 120 kDa, which likely accounted for the large increase in PHF-1 signal seen at mitosis. Although the size of the 120-kDa band is consistent with it being the high-molecular-weight form of tau, other antibodies to tau did not recognize it. Second, mitosis caused a hyperphosphorylation of the PHF-1 immunoreactive tau band normally seen at 50 kDa. In mitotic cells this band had an increased intensity and molecular weight. Alkaline phosphatase treatment abolished tau M(r) heterogeneity, verifying that the variations in mobility were due to phosphorylation. These data show that cell-cycle-dependent hyperphosphorylation of tau occurs in intact cells, and they support the hypothesis that aberrant activity of cell cycle-dependent kinases may contribute to tau phosphorylation and PHF formation in Alzheimer's disease. (C) 1994 Academic Press, Inc.
引用
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页码:185 / 194
页数:10
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