DETECTION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS GENOME WITH A BIOTINYLATED DNA PROBE GENERATED BY POLYMERASE CHAIN-REACTION

被引：4

作者：

FORGHANI, B

HURST, JW

SHELL, GR

机构：

[1] Viral and Rickettsial Disease Laboratory, Division of Laboratories, California State Department, Berkeley, CA 94704

来源：

MOLECULAR AND CELLULAR PROBES | 1991年 / 5卷 / 03期

关键词：

HIV; PCR; BIOTINYLATED DNA PROBE;

D O I：

10.1016/0890-8508(91)90044-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A non-radiolabelled DNA probe was developed for detection of the human immunodeficiency virus type 1 (HIV-1) genome using the polymerase chain reaction (PCR) technology. Primers amplifying a 395 base pair segment of a portion of the polymerase region of the HIV-1 genome were used both to amplify sample target DNA and to generate a biotinylated DNA probe used in Southern blot hybridization. This probe performed as well as one produced by nick translation using biotinylated nucleotides or an enzyme labelled oligonucleotide probe. © 1991.

引用

页码：221 / 228

页数：8

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