POLYACRYLAMIDE-MODIFIED POLYPROPYLENE HOLLOW FIBERS FOR CAPILLARY ELECTROPHORESIS

被引:21
作者
LIU, PZ
MALIK, A
KUCHAR, MCJ
LEE, ML
机构
[1] Department of Chemistry, Brigham Young University, Provo, Utah
关键词
CAPILLARY ELECTROPHORESIS; POLYMERIC HOLLOW FIBERS; GRAFT COPOLYMERIZATION; SURFACE MODIFICATION; POLYACRYLAMIDE COATING;
D O I
10.1002/mcs.1220060609
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Surface treatment methods were developed for bonding of a polyacrylamide layer on the inside surface of polypropylene hollow fiber columns for capillary electrophoresis. Details of different modification procedures involving surface activation and in situ polymerization, using dynamic and static coating of reagents, are described. Both linear and cross-linked polyacrylamide modified surfaces greatly improved the electrophoretic performance of the capillaries. The column efficiencies for model proteins reached as high as 370,000 theoretical plates on an 85 mu m i.d. polyacrylamide surface-treated polypropylene hollow fiber column. A variety of applications, including the separation of several very polar compounds at a pH of 10.6, were demonstrated. Reproducibility measurements were used to illustrate the performance and high pH stability of the resultant columns.
引用
收藏
页码:581 / 589
页数:9
相关论文
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