ASCORBYL FREE-RADICAL - A NONINVASIVE MARKER OF OXIDATIVE STRESS IN HUMAN OPEN-HEART-SURGERY

被引:55
作者
PIETRI, S
SEGUIN, JR
DARBIGNY, P
CULCASI, M
机构
[1] UNIV AIX MARSEILLE 1,SREP,CNRS,URA 1412,F-13397 MARSEILLE 13,FRANCE
[2] HOP ARNAUD VILLENEUVE,DEPT THORAC & CARDIOVASC SURG,MONTPELLIER,FRANCE
[3] HOP ARNAUD VILLENEUVE,DEPT CARDIOVASC PHYSIOL,MONTPELLIER,FRANCE
[4] INST IPSEN,PARIS 15,FRANCE
[5] CRIPDOM,DEPT PHARMACEUT CHEM,CASTELNAU LE LEZ,FRANCE
关键词
ASCORBATE; ASCORBYL FREE RADICAL; CARDIAC SURGERY; CLINICAL STUDY; ELECTRON SPIN RESONANCE; FREE RADICALS;
D O I
10.1016/0891-5849(94)90131-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To assess the development of oxidative stress in cardiac ischemia/reperfusion, the resulting depletion of plasma ascorbate was monitored by electron spin resonance spectroscopic detection of ascorbyl free radical (AFR) in a homogeneous group of 12 patients undergoing aortic valve replacement. Dimethyl sulfoxide (DMSO) was used as an enhancer and stabilizer for AFR in plasma separated from blood samples collected 15 min before incision, 10 min before aortic declamping, and sequentially during the initial 30 min of reperfusion. Plasma DMSO/AFR levels of patients were found to be significantly lower than in healthy subjects (-25%), further decreased upon ischemia (-35%), dropped to their lowest values within the first 10 min of reperfusion (-46%), and did not recover their initial values within 30 min following reflow. Cardiac index measurements revealed a still depressed heart function 4 h postdeclamping and a more delayed tissue injury was evidenced by cardiac myosin and myoglobin release in plasma. DMSO/AFR levels at early reperfusion were slightly (+12%) higher in plasma obtained from coronary sinus samples than in plasma from peripheral blood, suggesting an extra ascorbate release from the injured heart tissue. The close analogy between these results and the reported measurements of other plasma markers of oxidative stress, including ascorbate, indicates that the present method could be of great value in clinical practice.
引用
收藏
页码:523 / 528
页数:6
相关论文
共 25 条
  • [11] RELATIONSHIP OF PLASMA-LEVEL OF VITAMIN-C TO MORTALITY FROM ISCHEMIC-HEART-DISEASE
    GEY, KF
    STAHELIN, HB
    PUSKA, P
    EVANS, A
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1987, 498 : 110 - 123
  • [12] MONITORING OF OXIDATIVE FREE-RADICAL DAMAGE INVIVO - ANALYTICAL ASPECTS
    HAGEMAN, JJ
    BAST, A
    VERMEULEN, NPE
    [J]. CHEMICO-BIOLOGICAL INTERACTIONS, 1992, 82 (03) : 243 - 293
  • [13] THE ANTIOXIDANTS OF HUMAN EXTRACELLULAR FLUIDS
    HALLIWELL, B
    GUTTERIDGE, JMC
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 280 (01) : 1 - 8
  • [14] HUME R, 1972, BRIT HEART J, V34, P238, DOI 10.1136/hrt.34.3.238
  • [15] MCCORD JM, 1985, NEW ENGL J MED, V312, P159
  • [16] IRON-INDUCED ASCORBATE OXIDATION IN PLASMA AS MONITORED BY ASCORBATE FREE-RADICAL FORMATION - NO SPIN-TRAPPING EVIDENCE FOR THE HYDROXYL RADICAL IN IRON-OVERLOADED PLASMA
    MINETTI, M
    FORTE, T
    SORIANI, M
    QUARESIMA, V
    MENDITTO, A
    FERRARI, M
    [J]. BIOCHEMICAL JOURNAL, 1992, 282 : 459 - 465
  • [17] ANTIOXIDANT DEPLETION IN AORTIC CROSS-CLAMPING ISCHEMIA - INCREASE OF THE PLASMA ALPHA-TOCOPHERYL QUINONE ALPHA-TOCOPHEROL RATIO
    MURPHY, ME
    KOLVENBACH, R
    ALEKSIS, M
    HANSEN, R
    SIES, H
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 1992, 13 (02) : 95 - 100
  • [18] ASCORBYL FREE-RADICAL AS A RELIABLE INDICATOR OF FREE-RADICAL-MEDIATED MYOCARDIAL ISCHEMIC AND POSTISCHEMIC INJURY - A REAL-TIME CONTINUOUS-FLOW ESR STUDY
    PIETRI, S
    CULCASI, M
    STELLA, L
    COZZONE, PJ
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 193 (03): : 845 - 854
  • [19] REAL-TIME CONTINUOUS-FLOW SPIN TRAPPING OF HYDROXYL FREE-RADICAL IN THE ISCHEMIC AND POST-ISCHEMIC MYOCARDIUM
    PIETRI, S
    CULCASI, M
    COZZONE, PJ
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 186 (1-2): : 163 - 173
  • [20] INCREASED OXYGEN FREE-RADICAL ACTIVITY IN PATIENTS ON CARDIOPULMONARY BYPASS UNDERGOING AORTOCORONARY BYPASS-SURGERY
    PRASAD, K
    KALRA, J
    BHARADWAJ, B
    CHAUDHARY, AK
    [J]. AMERICAN HEART JOURNAL, 1992, 123 (01) : 37 - 45