MICRONUCLEI AND 3AB INDEX IN X-IRRADIATED HUMAN-LYMPHOCYTES IN G0 AND G1 PHASES

被引：6

作者：

CATENA, C ^{[1
]}

VILLANI, P ^{[1
]}

CONTI, D ^{[1
]}

RIGHI, E ^{[1
]}

机构：

[1] INFN,NATL LABS FRASCATI,OCCUPAT MED SERV,I-00044 FRASCATI,ITALY

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1994年 / 311卷 / 02期

关键词：

MICRONUCLEI; 3-AMINOBENZAMIDE; 3AB INDEX; LYMPHOCYTE CELL CYCLE; X-IRRADIATION;

D O I：

10.1016/0027-5107(94)90181-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We applied the cytokinesis-block micronucleus assay to observe the radiobiological response of human lymphocytes after X-ray treatment in the G0 and G1 phases. In addition, we used 3-aminobenzamide (3AB) to measure the 3AB index in the two phases. The experimental results show that at 2 Gy the MN yield and the 3AB index are dependent on the cell phase and show considerable inter-individual variability. The radiation-induced MN frequency obtained for 33 subjects is 0.470 +/- 0.063 for the G0 phase and 0.689 +/- 0.139 for the G1 phase; the 3AB index values are 0.326 +/- 0.144 and 0.067 +/- 0.058 for G0 and G1 phases, respectively. At the individual level, the 3AB index for the G1 phase correlates inversely with the cytogenetic effects observed in that phase. We discuss the possibility of applying the MN test combined with the 3AB index to lymphocytes at different phases to study the individual response to radiation (individual radiosensitivity).

引用

页码：231 / 237

页数：7

共 32 条

[11] POLY(ADP-RIBOSE)POLYMERASE - A PERPLEXING PARTICIPANT IN CELLULAR-RESPONSES TO DNA BREAKAGE [J].

CLEAVER, JE ;

MORGAN, WF .

MUTATION RESEARCH, 1991, 257 (01) :1-18

[12] PRODUCTION OF MICRONUCLEI FROM CHROMOSOME-ABERRATIONS IN IRRADIATED CULTURES OF HUMAN LYMPHOCYTES [J].

COUNTRYMAN, PI ;

HEDDLE, JA .

MUTATION RESEARCH, 1976, 41 (2-3) :321-331

[13] HUMAN PERIPHERAL-BLOOD LYMPHOCYTES FOR ANALYSIS OF CHROMOSOME-ABERRATIONS IN MUTAGEN TESTS [J].

EVANS, HJ ;

ORIORDAN, ML .

MUTATION RESEARCH, 1975, 31 (03) :135-148

[14] AGING INVIVO DOES NOT INFLUENCE MICRONUCLEUS INDUCTION IN HUMAN-LYMPHOCYTES BY X-IRRADIATION [J].

FENECH, M ;

MORLEY, AA .

MECHANISMS OF AGEING AND DEVELOPMENT, 1987, 39 (02) :113-119

[15]

FENECH M, 1985, MUTAT RES, V148, P99, DOI 10.1016/0027-5107(85)90212-X

[16] MEASUREMENT OF MICRONUCLEI IN LYMPHOCYTES [J].

FENECH, M ;

MORLEY, AA .

MUTATION RESEARCH, 1985, 147 (1-2) :29-36

[17]

HASHIMOTO Y, 1975, BLOOD, V45, P503

[18] THE EFFECT OF 3-AMINOBENZAMIDE ON THE FREQUENCY OF X-RAY-INDUCED OR NEUTRON-INDUCED CHROMOSOME-ABERRATIONS IN CYCLING OR NON-CYCLING HUMAN-LYMPHOCYTES [J].

HEARTLEIN, MW ;

PRESTON, RJ .

MUTATION RESEARCH, 1985, 148 (1-2) :91-97

[19] LACK OF SYNERGISTIC EFFECT BETWEEN X-RAY AND UV IRRADIATION ON FREQUENCY OF CHROMOSOME-ABERRATIONS IN PHA-STIMULATED HUMAN LYMPHOCYTES IN G1 STAGE [J].

HOLMBERG, M .

MUTATION RESEARCH, 1976, 34 (01) :141-147

[20] REPAIR OF IONIZING-RADIATION INDUCED DNA DAMAGE IN HUMAN LYMPHOCYTES [J].

LAVIN, MF ;

KIDSON, C .

NUCLEIC ACIDS RESEARCH, 1977, 4 (11) :4015-4022

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