MICRONUCLEI AND 3AB INDEX IN X-IRRADIATED HUMAN-LYMPHOCYTES IN G0 AND G1 PHASES

被引：6

作者：

CATENA, C ^{[1
]}

VILLANI, P ^{[1
]}

CONTI, D ^{[1
]}

RIGHI, E ^{[1
]}

机构：

[1] INFN,NATL LABS FRASCATI,OCCUPAT MED SERV,I-00044 FRASCATI,ITALY

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1994年 / 311卷 / 02期

关键词：

MICRONUCLEI; 3-AMINOBENZAMIDE; 3AB INDEX; LYMPHOCYTE CELL CYCLE; X-IRRADIATION;

D O I：

10.1016/0027-5107(94)90181-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We applied the cytokinesis-block micronucleus assay to observe the radiobiological response of human lymphocytes after X-ray treatment in the G0 and G1 phases. In addition, we used 3-aminobenzamide (3AB) to measure the 3AB index in the two phases. The experimental results show that at 2 Gy the MN yield and the 3AB index are dependent on the cell phase and show considerable inter-individual variability. The radiation-induced MN frequency obtained for 33 subjects is 0.470 +/- 0.063 for the G0 phase and 0.689 +/- 0.139 for the G1 phase; the 3AB index values are 0.326 +/- 0.144 and 0.067 +/- 0.058 for G0 and G1 phases, respectively. At the individual level, the 3AB index for the G1 phase correlates inversely with the cytogenetic effects observed in that phase. We discuss the possibility of applying the MN test combined with the 3AB index to lymphocytes at different phases to study the individual response to radiation (individual radiosensitivity).

引用

页码：231 / 237

页数：7

共 32 条

[21] THE INFLUENCE OF CELL-CYCLE KINETICS ON THE RADIOSENSITIVITY OF DOWNS-SYNDROME LYMPHOCYTES [J].

LEONARD, JC ;

MERZ, T .

MUTATION RESEARCH, 1983, 109 (01) :111-121

[22] GAMMA-RADIATION-INDUCED CHROMOSOMAL-ABERRATIONS IN HUMAN LYMPHOCYTES - DOSE-RATE EFFECTS IN STIMULATED AND NON-STIMULATED CELLS [J].

LINIECKI, J ;

BAJERSKA, A ;

WYSZYNSKA, K ;

CISOWSKA, B .

MUTATION RESEARCH, 1977, 43 (02) :291-304

[23] RECOVERY OF HUMAN-LYMPHOCYTES DAMAGED WITH GAMMA-RADIATION OR ENZYMATICALLY PRODUCED OXYGEN RADICALS - DIFFERENT EFFECTS OF POLY(ADP-RIBOSYL)POLYMERASE INHIBITORS [J].

MARINI, M ;

ZUNICA, G ;

TAMBA, M ;

COSSARIZZA, A ;

MONTI, D ;

FRANCESCHI, C .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1990, 58 (02) :279-291

[24]

NATARAJAN AT, 1982, DNA REPAIR CHROMOSOM, P47

[25] SCREENING HUMAN-POPULATIONS FOR CHROMOSOME-ABERRATIONS [J].

NORMAN, A ;

BASS, D ;

ROE, D .

MUTATION RESEARCH, 1985, 143 (03) :155-160

[26] 3-AMINOBENZAMIDE DOES NOT AFFECT X-RAY-INDUCED CYTOGENETIC DAMAGE IN G0 HUMAN-LYMPHOCYTES [J].

PANTELIAS, GE ;

POLITIS, G ;

SABANI, CD ;

WIENCKE, JK ;

MORGAN, WF .

MUTATION RESEARCH, 1986, 174 (02) :121-124

[27] CHROMOSOMAL RADIOSENSITIVITY OF DOWN SYNDROME LYMPHOCYTES AT DIFFERENT STAGES OF THE CELL-CYCLE [J].

SHAFIK, HM ;

AU, WW ;

LEGATOR, MS .

HUMAN GENETICS, 1988, 78 (01) :71-75

[28] POLY(ADENOSINEDIPHOSPHORIBOSE) POLYMERASE INHIBITORS STIMULATE UNSCHEDULED DEOXYRIBONUCLEIC-ACID SYNTHESIS IN NORMAL HUMAN-LYMPHOCYTES [J].

SIMS, JL ;

SIKORSKI, GW ;

CATINO, DM ;

BERGER, SJ ;

BERGER, NA .

BIOCHEMISTRY, 1982, 21 (08) :1813-1821

[29]

Sirover M.A., 1990, TRANSFORMATION HUMAN, P29

[30] LACK OF RECOVERY FROM RADIATION INDUCED CHROMOSOME DAMAGE IN G0 HUMAN LYMPHOCYTES [J].

STEFANES.DT ;

TEODORES.M ;

BRUCHER, J ;

POPESCU, HI .

EXPERIMENTAL CELL RESEARCH, 1972, 71 (01) :156-&

← 1 2 3 4 →