THE LEADER PEPTIDE OF COLICIN-V SHARES CONSENSUS SEQUENCES WITH LEADER PEPTIDES THAT ARE COMMON AMONG PEPTIDE BACTERIOCINS PRODUCED BY GRAM-POSITIVE BACTERIA

被引:174
作者
HAVARSTEIN, LS
HOLO, H
NES, IF
机构
[1] Laboratory Microbial Gene Technology, Agricultural University of Norway, N-1432 As
来源
MICROBIOLOGY-SGM | 1994年 / 140卷
关键词
COLICIN V; BACTERIOCIN; MICROCIN;
D O I
10.1099/13500872-140-9-2383
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Colicin V is a ribosomally synthesized antimicrobial peptide produced by Escherichia coli. Four recently characterized genes, arranged in two convergent operons on the plasmid pCoIV-K30. are required for colicin V synthesis, export and immunity. We report the purification and N-terminal amino acid sequencing of the colicin V protein. Our results demonstrate that the colicin V primary translation product, which consists of 103 amino acids, is proteolytically processed. A leader peptide, consisting of 15 amino acid residues, is removed from the N-terminus during maturation of colicin V. This leader peptide is not related to the N-terminal signal sequences which direct proteins across the cytoplasmic membrane via the Sec pathway. The molecular mass of colicin V, obtained by mass spectrometry analysis, showed that the peptide consists of only unmodified amino acids. The deduced amino acid sequence of the leader peptide was highly homologous to the N-terminal extensions found in non-lantibiotic, peptide bacteriocins produced by Grampositive bacteria. These findings strongly indicate that colicin V belongs to a family of small peptide bacteriocins that have been found previously only among the Gram-positive lactic acid bacteria.
引用
收藏
页码:2383 / 2389
页数:7
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