THE ELECTROSTATIC NATURE OF THE SPORE OF PASTEURIA-PENETRANS, THE BACTERIAL PARASITE OF ROOT-KNOT NEMATODES

被引:23
作者
AFOLABI, P
DAVIES, KG
OSHEA, PS
机构
[1] AFRC,INST ARABLE CROPS RES,DEPT ENTOMOL & NEMATOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
[2] UNIV ESSEX,DEPT CHEM & BIOCHEM,COLCHESTER CO4 3SQ,ESSEX,ENGLAND
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 79卷 / 03期
关键词
D O I
10.1111/j.1365-2672.1995.tb03133.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The response of spores of Pasteuria penetrans, the Gram-positive obligate nematode hyperparasite, was studied in a direct current electric field and monitored using a microscope attached to a video recorder apparatus. Fluorescence measurements were performed on the spore's surface using HEXCO as a fluorescent probe. The mobilities of the spores and fluorescence measurements were performed in different salt concentrations and at different pH values. The results showed a significant electronegative potential at the spore surface which was dependent on the pH, salt concentration and valency of the cation present in the electrolyte medium. The results of the fluorescence experiments using HEXCO correlated well with the results obtained from the electrophoretic mobility experiments. A polyclonal antiserum raised to spores of P. penetrans affected the surface charge density and the data presented suggest that electrostatic interactions may be important in the binding of spores to the nematode cuticle, The binding of HEXCO to hydrophobic sites on the spores' surfaces suggests the possibility of other attractive forces also being important in the binding process.
引用
收藏
页码:244 / 249
页数:6
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