HUMAN FETAL HEPATOCYTES RESPOND TO INFLAMMATORY MEDIATORS AND EXCRETE BILE

被引:16
作者
BAUER, J
LENGYEL, G
THUNG, SN
JONAS, U
GEROK, W
ACS, G
机构
[1] MT SINAI MED CTR, DEPT PATHOL, NEW YORK, NY 10029 USA
[2] MT SINAI MED CTR, DEPT BIOCHEM & NEOPLAST DIS, NEW YORK, NY 10029 USA
[3] UNIV FREIBURG, SCH MED, MED KLIN, W-7800 FREIBURG, GERMANY
[4] SEMMELWEIS UNIV MED, FAC MED, H-1085 BUDAPEST 8, HUNGARY
关键词
D O I
10.1016/0270-9139(91)92484-P
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Under strict observation of the ethical guidelines of the 1975 Declaration of Helsinki Human Research Committee, primary hepatocyte cultures were prepared from second-trimester fetal liver specimens. We have shown for the first time that fetal hepatocytes have the capacity to produce an acute-phase response on treatment with inflammatory mediators. Addition of interleukin-6 to the cultures resulted in strong induction of C-reactive protein and alpha-1-antichymotrypsin expression, whereas albumin expression was repressed. In contrast to interleukin-6, transforming growth factor-beta did not induce C-reactive protein expression. However, as in adult hepatocytes, fetal cells responded to transforming growth factor-beta by reduced albumin synthesis. We were able to show by virtue of fluorescein excretion into sealed clefts that fetal hepatocytes have the functional capacity to form bile. Our findings indicate that second-trimester hepatocytes can be regarded as fairly mature liver cells.
引用
收藏
页码:1131 / 1141
页数:11
相关论文
共 65 条
[11]   THE BIOLOGY OF CACHECTIN/TNF - A PRIMARY MEDIATOR OF THE HOST RESPONSE [J].
BEUTLER, B ;
CERAMI, A .
ANNUAL REVIEW OF IMMUNOLOGY, 1989, 7 :625-655
[12]   TISSUE-SPECIFIC EXPRESSION, DEVELOPMENTAL REGULATION, AND GENETIC-MAPPING OF THE GENE ENCODING CCAAT ENHANCER BINDING-PROTEIN [J].
BIRKENMEIER, EH ;
GWYNN, B ;
HOWARD, S ;
JERRY, J ;
GORDON, JI ;
LANDSCHULZ, WH ;
MCKNIGHT, SL .
GENES & DEVELOPMENT, 1989, 3 (08) :1146-1156
[13]   RADIOIMMUNOASSAY OF SERUM-ALBUMIN PRODUCED BY HUMAN EMBRYONIC LIVER IN MONOLAYER CULTURE [J].
BISSELL, DM ;
TILLES, JG .
JOURNAL OF CELL BIOLOGY, 1972, 53 (03) :819-+
[14]   TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) INHIBITS ALBUMIN SYNTHESIS IN NORMAL HUMAN HEPATOCYTES AND IN HEPATOMA HEPG2 CELLS [J].
BUSSO, N ;
CHESNE, C ;
DELERS, F ;
MOREL, F ;
GUILLOUZO, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 171 (02) :647-654
[15]   POSTNATAL REPRESSION OF THE ALPHA-FETOPROTEIN GENE IS ENHANCER INDEPENDENT [J].
CAMPER, SA ;
TILGHMAN, SM .
GENES & DEVELOPMENT, 1989, 3 (04) :537-546
[16]   INTERLEUKIN-6 IS THE MAJOR REGULATOR OF ACUTE PHASE PROTEIN-SYNTHESIS IN ADULT HUMAN HEPATOCYTES [J].
CASTELL, JV ;
GOMEZLECHON, MJ ;
DAVID, M ;
ANDUS, T ;
GEIGER, T ;
TRULLENQUE, R ;
FABRA, R ;
HEINRICH, PC .
FEBS LETTERS, 1989, 242 (02) :237-239
[17]   A LIVER-SPECIFIC FACTOR ESSENTIAL FOR ALBUMIN TRANSCRIPTION DIFFERS BETWEEN DIFFERENTIATED AND DEDIFFERENTIATED RAT HEPATOMA-CELLS [J].
CEREGHINI, S ;
BLUMENFELD, M ;
YANIV, M .
GENES & DEVELOPMENT, 1988, 2 (08) :957-974
[18]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[19]  
Ciliberto G., 1989, ACUTE PHASE PROTEINS, P29, DOI [10.1007/978-1-4471-1739-1_3, DOI 10.1007/978-1-4471-1739-1_3]
[20]   DEPENDENCE OF LIVER-SPECIFIC TRANSCRIPTION ON TISSUE ORGANIZATION [J].
CLAYTON, DF ;
HARRELSON, AL ;
DARNELL, JE .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (10) :2623-2632